Comparison of the beating of cis‐ and trans‐flagella of Chlamydomonas cells held on micropipettes
Comparison of the beating of cis‐ and trans‐flagella of Chlamydomonas cells held on micropipettes
复制标题
微量移液器上衣藻细胞顺式和反式鞭毛跳动的比较
DOI：
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发表时间：
1987
期刊：
影响因子：
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通讯作者：
W. Nultsch
中科院分区：
文献类型：
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作者：
U. Rüffer;W. Nultsch
以500帧/秒的速度对吸到微量移液管上的衣原体细胞进行拍摄，并分析其向前跳动模式。与自由游动细胞的比较显示，被吸细胞的鞭毛以正常的三维方式跳动，其跳动频率与自由游动细胞的频率相对应。大多数心跳是同步的。但不对称;顺式和反式鞭毛似乎以稍微不同的方式跳动。一些细胞在整个过程中同步跳动，但大多数同步被一个单一的同步或不间断的同步中断，这是由trans-fo-flagellum的瞬时加速引起的。只有很少顺式和反式鞭毛有不同的，但恒定的拍频。衣原体的螺旋游泳更可能是由于两个鞭毛的节拍不对称而不是节拍频率的差异。在我们的记录中，柱头位于螺旋状游动通道的内侧。
Chlamydomonas cells sucked onto micropipettes were filmed at 500 frames/sec and analyzed as to their forward beating mode. A comparison with freely swimming cells revealed that the flagella of the sucked cells beat in a normal threedimensional manner, with beat frequencies that correspond to those of freely swimming cells. Most beats were synchronous. but not symmetrical; cis- and trans-flagellum appear to beat in a slightly different manner. Some cells beat synchronously throughout, but mostly synchrony was interrupted by a single asynchrony or up to incessant asynchronies, caused by transient accelerations of the trans- (fo-) flagellum. Only rarely did cis- and trans-flagella have different but constant beat frequencies. Helical swimming of Chlamydomonas more likely is due to the beat asymmetries of the two flagella than to differences of beat frequencies. In our records, the stigma is on the inside of the helical swimming path.