Continuous cytotoxic T cell lines reactive against murine plasmacytoma tumor-associated antigens.
Continuous cytotoxic T cell lines reactive against murine plasmacytoma tumor-associated antigens.
复制标题
对鼠浆细胞瘤肿瘤相关抗原具有反应性的连续细胞毒性 T 细胞系。
作者：
J. Giorgi;N. Warner
通过在体外使正常 BALB/c 和 (C57BL/6 X BALB/c)F1 脾淋巴细胞对 BALB/c 浆细胞瘤 (PCT) MPC-11 敏感，产生针对鼠肿瘤相关抗原 (TAA) 的连续细胞毒性 T 细胞系。这些细胞毒性 T 细胞系 (CTLL) 在来自有丝分裂原刺激的大鼠脾细胞培养物的 T 细胞生长因子中维持超过 1 年。这些细胞系针对免疫肿瘤以及针对选定数量的其他肿瘤细胞系具有高度细胞毒性，并且其中3个细胞系针对各种肿瘤细胞靶标的反应模式表明这些细胞系各自识别不同的PCT TAA。识别的抗原包括MHC限制性PCT抗原、PCT和T淋巴瘤共有的抗原以及MPC-11独特的PCT抗原。 CTLL 群体细胞表面标志物表达的表型特征表明，它们在分化状态方面很大程度上是同质的，并在细胞表面表达 Thy-1.2、Lyt-2、Lyt-3.2 和 T30 抗原。此外，其中 1 个品系表现出 Lyt-1 的可变低水平表达。因此，这些群体具有成熟T细胞的特征，并且不表达其他淋巴细胞亚群特征的细胞表面标志物。
Continuous cytotoxic T cell lines against murine tumor-associated antigens (TAA) were generated from normal BALB/c and (C57BL/6 X BALB/c)F1 spleen lymphocytes by sensitizing these cells in vitro to the BALB/c plasmacytoma (PCT) MPC-11. These cytotoxic T cell lines (CTLL) were maintained for periods of over 1 yr in T cell growth factor from cultures of mitogen-stimulated rat spleen cells. The lines were highly cytotoxic against the immunizing tumor, as well as against a select number of other tumor cell lines, and the patterns of reactivity of 3 of these lines against the various tumor cell targets indicated that these lines each recognized different PCT TAA. The antigens recognized included an MHC-restricted PCT antigen, an antigen shared by PCT and T lymphomas, and an MPC-11 unique PCT antigen. phenotypic characterization of the CTLL populations for cell surface marker expression indicated that they were largely homogeneous with respect to their differentiation state, and expressed Thy-1.2, Lyt-2, Lyt-3.2, and T30 antigens on their cell surface. In addition, 1 of the lines demonstrated variable low-level expression of Lyt-1. Thus, the populations were characteristics of mature T cells, and did not express cell surface markers characteristic of other lymphocyte subpopulations.