Edible mushroom (Flammulina velutipes) as biosource for silver nanoparticles: from synthesis to diverse biomedical and environmental applications
Edible mushroom (Flammulina velutipes) as biosource for silver nanoparticles: from synthesis to diverse biomedical and environmental applications
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DOI：
10.1088/1361-6528/abc2eb
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发表时间：
2021-02-05
期刊：
影响因子：
3.5
通讯作者：
Redaina
中科院分区：
文献类型：
--
作者：
Faisal, Shah;Khan, Muhammad Aslam;Redaina
目前的研究报告了先进的、生态友好的、生物合成的银NPs，以金针菇为生物来源，用于多种生物医学和环境应用。在这项研究中，利用一种简单的水萃取物，在纳米尺度上将AgNO3还原成稳定的单质银（Ag-0）。NPs的平均尺寸为21.4 nm，形貌为球形，具有较高的稳定性和纯度。所表征的纳米颗粒被广泛应用于生物医学领域，包括杀菌、杀真菌、杀利什曼尼、体外抗阿尔茨海默病、抗氧化、抗糖尿病和生物相容性研究。我们的研究结果表明，镰刀菌介导的AgNPs对耐多药菌株和孢子形成真菌菌株具有较高的活性。所有测试的尿路感染细菌分离株均对非包被抗生素耐药，但应用1%的合成AgNPs，测试抗生素的杀菌潜力增强。NPs对热带利什曼原虫也表现出剂量依赖性的细胞毒潜能，对原鞭毛虫的LC50为248 μ g ml(-1)，对无鞭毛虫的LC50为251 μ g ml（-1）。此外，由于对α -淀粉酶、α -葡萄糖苷酶、乙酰胆碱酯酶（AChE）和丁基胆碱酯酶（BChE）有明显的抑制作用，因此具有抗阿尔茨海默病和抗糖尿病的活性。此外，发现颗粒对人体红细胞具有显著的生物相容性。生物合成的AgNPs作为光催化剂，在140 min内对靛蓝胭脂红染料的降解率达到98.2%。由于其生态友好的合成、生物安全的性质和优异的物理化学性质，F. velutipes AgNPs可以作为多方面生物医学和环境应用的新候选物而被开发。
The current study reports advanced, ecofriendly and biosynthesized silver NPs for diverse biomedical and environmental applications using Flammulina velutipes as biosource. In the study, a simple aqueous extract of F. velutipes was utilized to reduce the AgNO3 into stable elemental silver (Ag-0) at a nanometric scale. The NPs had average size of 21.4 nm, spherical morphology, and were highly stable and pure. The characterized nanoparticles were exploited for a broad range of biomedical applications including bacteriocidal, fungicidal, leishmanicidal, in vitro antialzheimer's, antioxidant, anti-diabetic and biocompatibility studies. Our findings showed that F. velutipes mediated AgNPs exhibited high activity against MDR bacterial strains and spore forming fungal strains. All the tested urinary tract infection bacterial isolates, were resistant to non-coated antibiotics but by applying 1% of the synthesized AgNPs, the bactericidal potential of the tested antibiotics enhanced manifolds. The NPs also exhibited dose-dependent cytotoxic potential against Leishmania tropica with significant LC50 of 248 mu g ml(-1) for promastigote and 251 mu g ml(-1) for amastigote forms of the parasite. Furthermore, promising antialzheimer and antidiabetic activities were observed as significant inhibition of alpha-amylase, alpha-glucosidase, acetylcholinesterase (AChE) and butrylcholineterase (BChE) were noted. Moreover, remarkable biocompatible nature of the particles was found against human red blood cells. The biosynthesized AgNPs as photocatalyst, also resulted in 98.2% degradation of indigo carmine dye within 140 min. Owing to ecofriendly synthesis, biosafe nature and excellent physicochemical properties F. velutipes AgNPs can be exploited as novel candidates for multifaceted biomedical and environmental applications.