Single-Lumen and Multi-Lumen Poly(Ethylene Glycol) Nerve Conduits Fabricated by Stereolithography for Peripheral Nerve Regeneration In Vivo
Single-Lumen and Multi-Lumen Poly(Ethylene Glycol) Nerve Conduits Fabricated by Stereolithography for Peripheral Nerve Regeneration In Vivo
复制标题
DOI：
10.1055/s-0034-1395415
复制
发表时间：
2015-06-01
影响因子：
2.1
通讯作者：
Evans, Gregory R. D.
中科院分区：
文献类型：
--
作者：
Evangelista, Maristella S.;Perez, Mireya;Evans, Gregory R. D.
背景周围神经损伤后使用神经导管促进神经再生仅限于缺损小于3cm。本研究的目的是确定新型单腔和多腔聚乙二醇（PEG）导管通过立体光面术制造促进周围神经再生的能力。材料与方法选取8只坐骨神经尖锐横断损伤的Sprague Dawley大鼠，随机分为单腔或多腔PEG导管架桥。5周后取坐骨神经和导管标本，分析轴突数、髓鞘厚度、纤维直径、g比。对侧完整神经也被切除以作比较。结果4例单腔导管中3例神经再生，4例多腔导管中1例神经再生。单腔再生神经的轴突数量接近对侧完整神经，分别为4,492 +/- 2,810.0和6,080 +/- 627.9纤维/mm(2)。与完整神经相比，单腔导管中小纤维的百分比更高，而自体神经中的髓鞘厚度和g-比值始终更高。通过多腔导管的轴突再生受到严重限制。结论单腔立体平铺造影术制造的聚乙二醇神经导管促进神经再生，再生轴突数量接近正常神经。多腔导管显示神经再生明显减少，可能是由于导管抑制生长的物理特性。需要进一步的研究来比较两种导管在功能恢复方面的效果，并阐明它们在神经再生潜能方面差异的原因。
Background The use of nerve conduits to facilitate nerve regrowth after peripheral nerve injury is limited to defects less than 3 cm. The purpose of this study is to determine the capability of novel single and multi-lumen poly(ethylene glycol) (PEG) conduits manufactured by stereolithography to promote peripheral nerve regeneration.Materials and Methods Eight Sprague Dawley rats with sharp transection injuries of the sciatic nerve were randomly assigned to receive single-lumen or multi-lumen PEG conduits to bridge a 10-mm gap. Sciatic nerve and conduit samples were harvested after 5 weeks, and axon number, myelin thickness, fiber diameter, and g-ratio were analyzed. The contralateral intact nerve was also harvested for comparison.Results Partial nerve regeneration was observed in three out of four single-lumen conduits and one out of four multi-lumen conduits. Axon number in the single-lumen regenerated nerve approached that of the contralateral intact nerve at 4,492 +/- 2,810.0 and 6,080 +/- 627.9 fibers/mm(2), respectively. The percentage of small fibers was greater in the single-lumen conduit compared with the intact nerve, whereas myelin thickness and g-ratio were consistently greater in the autologous nerve. Axon regrowth through the multi-lumen conduits was severely limited.Conclusion Single-lumen stereolithography-manufactured PEG nerve conduits promote nerve regeneration, with regenerating axon numbers approaching that of normal nerve. Multi-lumen conduits demonstrated significantly less nerve regeneration, possibly due to physical properties of the conduit inhibiting growth. Further studies are necessary to compare the efficacy of the two conduits for functional recovery and to elucidate the reasons underlying their differences in nerve regeneration potential.