Single-cell force spectroscopy
Single-cell force spectroscopy
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DOI：
10.1242/jcs.030999
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发表时间：
2008-06-01
影响因子：
4
通讯作者：
Muller, Daniel J.
中科院分区：
文献类型：
--
作者：
Helenius, Jonne;Heisenberg, Carl-Philipp;Muller, Daniel J.
细胞之间以及细胞与细胞外基质的受控粘附对于组织发育和维持至关重要。已经开发了许多测定来量化细胞粘附。其中，使用原子力显微镜（AFM）的单细胞力谱（SCFS）最近已经建立。该测定允许在接近生理条件下研究活细胞的粘附。AFM的这种实现允许对细胞进行无与伦比的空间和时间控制，以及高度定量的力致动和力测量，其足够敏感以表征单分子的相互作用。因此，不仅可以研究整体细胞粘附，而且可以研究单个粘附-受体-配体相互作用的性质。在这里，我们描述了目前的实现和应用的SCFS，以及潜在的陷阱，并概述了如何发展将提供洞察力，能量学和动力学的细胞粘附过程。
The controlled adhesion of cells to each other and to the extracellular matrix is crucial for tissue development and maintenance. Numerous assays have been developed to quantify cell adhesion. Among these, the use of atomic force microscopy (AFM) for single-cell force spectroscopy (SCFS) has recently been established. This assay permits the adhesion of living cells to be studied in near-physiological conditions. This implementation of AFM allows unrivaled spatial and temporal control of cells, as well as highly quantitative force actuation and force measurement that is sufficiently sensitive to characterize the interaction of single molecules. Therefore, not only overall cell adhesion but also the properties of single adhesion-receptor-ligand interactions can be studied. Here we describe current implementations and applications of SCFS, as well as potential pitfalls, and outline how developments will provide insight into the forces, energetics and kinetics of cell-adhesion processes.