Arabidopsis GLAUCE promotes fertilization-independent endosperm development and expression of paternally inherited alleles
Arabidopsis GLAUCE promotes fertilization-independent endosperm development and expression of paternally inherited alleles
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DOI：
10.1242/dev.007310
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发表时间：
2007-11-15
期刊：
影响因子：
4.6
通讯作者：
Sundaresan, Venkatesan
中科院分区：
文献类型：
--
作者：
Ngo, Quy A.;Moore, James M.;Sundaresan, Venkatesan
有性繁殖植物的早期种子发育需要母本和父本基因组，但受母本影响显著。从拟南芥Ds转座子插入系群体中分离到一个新的配子体母源效应突变体-Glc。glc突变是由Ds插入位点的缺失引起的，GLC的分子身份尚不清楚。GLC胚可以在没有胚乳的情况下发育到球形阶段，并且GLC中央细胞似乎未受精。GLC抑制在不依赖受精的种子（FIS）类突变体中观察到的自主胚乳发育。glc在受精种子中对MEA（fis类突变体之一）也是上位性的，并且对于PHE 1（MEA的受抑制的下游靶标）的双亲胚胎表达是必需的。此外，母本GLC功能是父本胚胎表达核糖体蛋白基因RPS 5a和AMP脱氨酶基因FAC 1所必需的，这两个基因对早期胚胎和胚乳发育都是必需的。这些结果表明，来自雌配子体的因子激活了受精种子的父本基因组的一个子集。
Early seed development of sexually reproducing plants requires both maternal and paternal genomes but is prominently maternally influenced. A novel gametophytic maternal-effect mutant defective in early embryo and endosperm development, glauce ( glc), has been isolated from a population of Arabidopsis Ds transposon insertion lines. The glc mutation results from a deletion at the Ds insertion site, and the molecular identity of GLC is not known. glc embryos can develop up to the globular stage in the absence of endosperm and glc central cells appear to be unfertilized. glc suppresses autonomous endosperm development observed in the fertilization-independent seed ( fis) class mutants. glc is also epistatic to mea, one of the fis class mutants, in fertilized seeds, and is essential for the biparental embryonic expression of PHE1, a repressed downstream target of MEA. In addition, maternal GLC function is required for the paternal embryonic expression of the ribosome protein gene RPS5a and the AMP deaminase gene FAC1, both of which are essential for early embryo and endosperm development. These results indicate that factors derived from the female gametophyte activate a subset of the paternal genome of fertilized seeds.