Yeast adapt to near-freezing temperatures by STRE/Msn2,4-dependent induction of trehalose synthesis and certain molecular chaperones
Yeast adapt to near-freezing temperatures by STRE/Msn2,4-dependent induction of trehalose synthesis and certain molecular chaperones
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DOI：
10.1016/s1097-2765(04)00148-0
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发表时间：
2004-03-26
期刊：
影响因子：
16
通讯作者：
Goldberg, AL
中科院分区：
文献类型：
--
作者：
Kandror, O;Bretschneider, N;Goldberg, AL
事实上，我们对真核细胞的生化适应性一无所知，这些生物适应性可能会提高真核细胞在低温或冷冻条件下的存活率。在这里，我们展示了酵母的适应性反应，它在10摄氏度以下被激活，并增加了对低温和冷冻的耐受性。这种反应涉及化学伴侣海藻糖的急剧积累和海藻糖合成酶（Tps1，Tps2）和某些热休克蛋白（Hsp104，Hsp42，Hsp12，Ssa4）的诱导。这些蛋白质的mRNA在10 ℃以下甚至在0 ℃时急剧增加。它们的表达需要Msn2，4转录因子，但也涉及显着的mRNA稳定。回到30 ℃后，TPS1、TPS2和HSP 104 mRNA、海藻糖水平和冷冻耐受性在几分钟内急剧下降。缺乏海藻糖或Msn2，4的突变体在0 ℃和冷冻时死亡更快。因此，低于WC时，酵母表现出适应性反应，在自然环境和实验室冰箱中经常遇到的低温或冷冻温度下维持生存能力。
Virtually nothing is known about the biochemical adaptations in eukaryotic cells that may enhance survival at low temperatures or upon freezing. Here we demonstrate an adaptive response in yeast that is activated below 10degreesC and increases tolerance to low temperatures and freezing. This response involves a dramatic accumulation of the chemical chaperone trehalose and induction of trehalose-synthesizing enzymes (Tps1, Tps2) and certain heat shock proteins (Hsp104, Hsp42, Hsp12, Ssa4). mRNAs for these proteins increase dramatically below 10degreesC and even at 0degreesC. Their expression requires Msn2,4 transcription factors but also involves marked mRNA stabilization. Upon return to 30degreesC, TPS1, TPS2, and HSP104 mRNAs, trehalose levels and tolerance to freezing fall dramatically within minutes. Mutants lacking trehalose or Msn2,4 die more rapidly at 0degreesC and upon freezing. Thus, below WC, yeastshow an adaptive response that sustains viability at low or freezing temperatures, which are commonly encountered in natural environments and laboratory refrigerators.