Dehydroepiandrosterone protects bovine retinal capillary pericytes against glucose toxicity
Dehydroepiandrosterone protects bovine retinal capillary pericytes against glucose toxicity
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DOI：
10.1677/joe.0.1580021
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发表时间：
1998-07-01
影响因子：
4
通讯作者：
Boccuzzi, G
中科院分区：
文献类型：
--
作者：
Brignardello, E;Beltramo, E;Boccuzzi, G
周细胞丢失是糖尿病视网膜病变的早期特征，是糖尿病视网膜病变发展的关键一步。本研究旨在探讨脱氢表雄酮（DHEA）对视网膜毛细血管周细胞葡萄糖毒性的影响。牛视网膜周细胞（BRP）在高葡萄糖浓度下培养，有或没有脱氢表雄酮。培养4 d后，高葡萄糖浓度显著降低了BRP的数量。在培养基中加入DHEA逆转了高葡萄糖的不良影响：在DHEA浓度为10 nmol/l时，BRP增殖部分恢复，在DHEA浓度等于或大于100 nmol/l时，BRP增殖完全恢复。在生理葡萄糖浓度下，脱氢表雄酮对BRP生长无影响。数据显示，脱氢表雄酮的浓度与人类血浆中的浓度相似，可以保护BRP免受葡萄糖毒性。这种影响似乎是DHEA特有的，因为它的代谢物，5-烯雄烯-3 β，17 - β -二醇，二氢睾酮和雌二醇在正常或高糖培养基中不会改变BRP的生长。各种证据表明脱氢表雄酮的抗氧化特性与其对葡萄糖引起的BRP毒性的保护作用有关。
Pericyte loss is an early feature of diabetic retinopathy and represents a key step in the progression of this disease. This study aimed to evaluate the effect of dehydroepiandrosterone (DHEA) on glucose toxicity in retinal capillary pericytes. Bovine retinal pericytes (BRP) were cultured in a high glucose concentration, with or without DHEA. After 4 days of incubation the number of BRP was significantly reduced by the high glucose concentration. The addition of DHEA to the medium reversed the adverse effect of high glucose: BRP proliferation partially recovered in the presence of 10 nmol/l DHEA, and completely recovered in the presence of DHEA at concentrations equal to or greater than 100 nmol/l. At physiological glucose concentrations, DHEA had no effect on BRP growth. Data show that DHEA, at concentrations similar to those found in human plasma, protects BRP against glucose toxicity. This effect seems specific for DHEA, since its metabolites, 5-en-androstene-3 beta,17 beta-diol, dihydrotestosterone and estradiol did not alter BRP growth in normal or high glucose media. Various pieces of evidence link the antioxidant properties of DHEA to its protective effect on glucose-induced toxicity in BRP.