Adiponectin regulates functions of gingival fibroblasts and periodontal ligament cells
Adiponectin regulates functions of gingival fibroblasts and periodontal ligament cells
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DOI：
10.1111/j.1600-0765.2012.01467.x
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发表时间：
2012-10-01
影响因子：
3.5
通讯作者：
Murakami, S.
中科院分区：
文献类型：
--
作者：
Iwayama, T.;Yanagita, M.;Murakami, S.
Iwayama T，Yanagita M，Mori K，Sawada K，Ozasa M，Kubota M，三木K，Kojima Y，Takedachi M，Kitamura M，Shimabukuro Y，Hashikawa T，Murakami S.脂联素对牙龈成纤维细胞和牙周膜细胞功能的调节。J Periodont Res 2012; 47：563571. (C)2012 John Wiley & Sons A/S背景与目的：脂联素是一种由脂肪细胞组成性产生的细胞因子，通过靶向多种细胞类型发挥多种生物学功能。然而，脂联素对原代牙龈成纤维细胞和牙周膜细胞的影响尚不清楚。因此，我们研究脂联素对牙龈成纤维细胞和牙周膜细胞的影响。材料和方法：采用RT-PCR和Western blotting方法检测人牙龈成纤维细胞（HGFs）、小鼠牙龈成纤维细胞（MGFs）和人牙周膜细胞（HPDL）脂联素受体（AdipoR 1和AdipoR 2）的表达。在存在或不存在脂联素的情况下，用白细胞介素（IL）-1 β刺激HGF和MGFs，并分别通过实时PCR和ELISA测量IL-6和IL-8在mRNA和蛋白水平上的表达。此外，MGFs中的小干扰RNA（siRNA）用于敲低小鼠AdipoR 1和AdipoR 2的表达。实时荧光定量PCR检测脂联素对碱性磷酸酶（ALP）和Runx 2基因表达的影响。茜素红染色显示脂联素处理的HPDL细胞形成矿化结节。结果：AdipoR 1和AdipoR 2在HGFs、MGFs和HPDL细胞中均呈组成性表达。脂联素降低IL-1 β刺激的HGF和MGFs中IL-6和IL-8的表达。在MGFs中的AdipoR 1 siRNA揭示了脂联素对IL-6表达减少的作用可能是通过AdipoR 1介导的。脂联素处理HPDL细胞可促进ALP和Runx 2 mRNA的表达，上调ALP活性。此外，脂联素促进HPDL细胞矿化结节的形成。结论：我们的观察表明，脂联素对HGFs和MGFs具有抗炎作用，并促进HPDL细胞成骨细胞的活性。我们的结论是，脂联素具有强大的有益功能，以维持牙周健康的稳态，改善牙周病变，并有助于伤口愈合和组织再生。
Iwayama T, Yanagita M, Mori K, Sawada K, Ozasa M, Kubota M, Miki K, Kojima Y, Takedachi M, Kitamura M, Shimabukuro Y, Hashikawa T, Murakami S. Adiponectin regulates functions of gingival fibroblasts and periodontal ligament cells. J Periodont Res 2012; 47: 563571. (C) 2012 John Wiley & Sons A/S Background and Objective: Adiponectin is a cytokine constitutively produced by adipocytes and exhibits multiple biological functions by targeting various cell types. However, the effects of adiponectin on primary gingival fibroblasts and periodontal ligament cells are still unexplored. Therefore, we investigated the effects of adiponectin on gingival fibroblasts and periodontal ligament cells. Material and Methods: The expression of adiponectin receptors (AdipoR1 and AdipoR2) on human gingival fibroblasts (HGFs), mouse gingival fibroblasts (MGFs) and human periodontal ligament (HPDL) cells was examined using RT-PCR and western blotting. HGFs and MGFs were stimulated with interleukin (IL)-1 beta in the presence or absence of adiponectin, and the expression of IL-6 and IL-8 at both mRNA and protein levels was measured by real-time PCR and ELISA, respectively. Furthermore, small interfering RNAs (siRNAs) in MGFs were used to knock down the expression of mouse AdipoR1 and AdipoR2. The effects of adiponectin on the expression of alkaline phosphatase (ALP) and runt-related transcription factor 2 (Runx2) genes were evaluated by real-time PCR. Mineralized nodule formation of adiponectin-treated HPDL cells was revealed by Alizarin Red staining. Results: AdipoR1 and AdipoR2 were expressed constitutively in HGFs, MGFs and HPDL cells. Adiponectin decreased the expression of IL-6 and IL-8 in IL-1 beta-stimulated HGFs and MGFs. AdipoR1 siRNA in MGFs revealed that the effect of adiponectin on reduction of IL-6 expression was potentially mediated via AdipoR1. Adiponectin-treated HPDL cells promoted the expression of ALP and Runx2 mRNAs and up-regulated ALP activity. Furthermore, adiponectin enhanced mineralized nodule formation of HPDL cells. Conclusion: Our observations demonstrate that adiponectin exerts anti-inflammatory effects on HGFs and MGFs, and promotes the activities of osteoblastogenesis of HPDL cells. We conclude that adiponectin has potent beneficial functions to maintain the homeostasis of periodontal health, improve periodontal lesions, and contribute to wound healing and tissue regeneration.