Two additive QTLs conferring broad-spectrum resistance in potato to Globodera pallida are localized on resistance gene clusters
Two additive QTLs conferring broad-spectrum resistance in potato to Globodera pallida are localized on resistance gene clusters
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DOI：
10.1007/s001220051588
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发表时间：
2000-11-01
影响因子：
5.4
通讯作者：
Bakker, J
中科院分区：
文献类型：
--
作者：
van der Voort, JR;van der Vossen, E;Bakker, J
马铃薯对马铃薯胞囊线虫病的广谱抗性是一种复杂的遗传性状。然而，在本文中，我们表明，通过使用一组选定的PCN测试人群，广谱抗球蝇种苍白可以完全归因于两个位点的行动：Gpa 5和Gpa 6。这些基因座很容易通过基于mio步骤的策略进行定位。首先，通过使用覆盖马铃薯基因组大部分的AFLP标记的在线目录（http：//www.spg.nl/pv/aflp/catalog.htm）来评估两个基因座的染色体定位。随后，通过基于RFLP插入序列的CAPS标记来鉴定两个基因座的染色体区域。Gpa 5基因座解释了至少61%的遗传变异。该基因座定位于5号染色体上的一个区域，该区域先前已显示出对病毒（Nb，Rx 2）、真菌（R1）和非节段性（Gpa，Grp 1）病原体具有抗性因子。Gpa 6位点对抗性的影响较小（24%），并与Gpa 5具有相加作用。有趣的是，Gpa 6基因座映射到9号染色体上的一个区域，在同源番茄基因组中，病毒抗性基因Sw-5作为抗性基因簇的一部分存在于该区域。在马铃薯中，在该地区附近已经报道了对马铃薯X病毒的抗性。Gpa 6的图谱定位表明在马铃薯第9染色体长臂末端存在一个抗病基因簇。
Broad-spectrum resistance in potato to the potato cyst nematode (PCN) is commonly regarded as a complex inherited trait. Yet, in this paper we show that, by use of a selected set of PCN test populations, broad-spectrum resistance to the species Globodera pallida can be fully ascribed to the action of two loci: Gpa5 and Gpa6. These loci were readily mapped by means of a strategy based on mio steps. Firstly, the chromosomal localization of both loci was assessed by use of an online catalogue of AFLP markers covering a substantial part of the potato genome (http://www.spg.wau.nl/pv/aflp/catalog.htm). Subsequently the chromosomal regions of both loci were identified by means of CAPS markers based on RFLP insert sequences. Locus Gpa5 explains at least 61% of the genetic variation. This locus maps to chromosome 5 on a region which has previously been shown to harbor resistance factors to viral (Nb, Rx2), fungal (R1) and nematodal (Gpa, Grp1) pathogens. The Gpa6 locus exhibits a minor effect on the resistance (24%) and acts additively to Gpa5. Interestingly, the Gpa6 locus maps to a region on chromosome 9 where, in the homoeologous tomato genome, the virus resistance gene Sw-5 resides as part of a resistance gene cluster. in potato, resistance to potato virus X has been reported in the vicinity of this region. The map location of Gpa6 indicates the presence of a resistance gene cluster at the end of the long arm of chromosome 9 of potato.