PicU, a second serine protease autotransporter of uropathogenic Escherichia coli
PicU, a second serine protease autotransporter of uropathogenic Escherichia coli
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DOI：
10.1016/s0378-1097(03)00862-0
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发表时间：
2004-01-15
影响因子：
2.1
通讯作者：
Henderson, IR
中科院分区：
文献类型：
--
作者：
Parham, NJ;Srinivasan, U;Henderson, IR
大肠杆菌是尿路感染（UTI）的主要病原。与腹泻性大肠杆菌菌株一样，尿路致病性分离株具有毒力决定因素，使其区别于共生菌株，并使其产生与尿路感染相关的临床表现。一些自体转运蛋白与大肠杆菌和其他革兰氏阴性菌引起疾病的能力有关。最近，我们描述了尿路致病性大肠杆菌（UPEC）菌株中存在Sat，这是肠杆菌科（串串）亚家族丝氨酸蛋白酶自转运蛋白的一种毒素。利用通过自转运蛋白途径分泌的蛋白质的共同特征，我们从UPEC CFT073的基因组序列数据中鉴定了9个额外的自转运蛋白。令人惊讶的是，发现了两个额外的成员。其中一种被命名为PicU的蛋白与福氏志贺氏菌和肠聚集性大肠杆菌中发现的Pic蛋白同源。表达了PicU蛋白并对其功能活性进行了研究。(C) 2003欧洲微生物学会联合会。Elsevier B.V.版权所有。
Escherichia coli is the major aetiological agent of urinary tract infections (UTI). Like diarrhoeagenic strains of E coli, uropathogenic isolates possess virulence determinants that distinguish them from commensal strains and allow them to produce the clinical manifestations associated with UTI. Several autotransporter proteins have been associated with the ability of E coli, and other Gram-negative bacteria, to cause disease. Recently, we described the existence within uropathogenic E. coli (UPEC) strains of Sat, a toxin of the serine protease autotransporter of Enterobacteriaceae (SPATE) subfamily. Using features common to proteins secreted via the autotransporter pathway we have identified nine additional autotransporter proteins from the genomic sequence data of UPEC CFT073. Surprisingly, two additional members of the SPATE subfamily were identified. One protein, designated PicU, was homologous to the Pic protein identified in Shigella flexneri and enteroaggregative E. coli. The PicU protein was expressed and investigated for functional activity. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.