Ultrastructure of the dentin-adhesive interface after acid-base challenge.
Ultrastructure of the dentin-adhesive interface after acid-base challenge.
复制标题
酸碱挑战后牙本质-粘合剂界面的超微结构。
DOI：
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发表时间：
2004
影响因子：
3.3
通讯作者：
J. Tagami
中科院分区：
文献类型：
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作者：
S. Tsuchiya;T. Nikaido;H. Sonoda;R. Foxton;J. Tagami
目的
观察粘结剂修复体周围酸性和碱性化学物质体外序贯冲击后牙本质-粘结剂界面的超微结构。
材料和方法
在牛根牙本质中制备盒状空洞，修复方式分别为：反应剂粘结剂和反应剂糊剂(RB/RP)、Clearfi SE粘结剂和Clearfil AP-X(SE/APX)、ABF(实验性)和APX(ABF/APX)、Single Bond和APX(SB/APX)。将标本在水中保存1周后，将顺序浸泡检测的粘结完整性置于人工脱矿液(pH 4.5)中20min和5%NaOCl中20min。标本经切片、抛光、Ar离子酸蚀7min，镀金后扫描电子显微镜观察牙本质-粘附面。
结果
形态观察结果显示，牙本质与粘结剂粘结紧密。对于释氟修复体Rb/Rp，在修复体附近明显观察到一层厚厚的耐酸层；然而，粘结树脂Rb在酸碱挑战下部分降解。SE/APX、ABF/APX和SB/APX的粘接树脂具有较好的耐酸碱性能。使用自酸蚀粘结剂SE和ABF，在混合层下观察到一条厚度小于1微米的耐酸碱牙本质薄带。在湿法粘接系统SB的作用下，杂化层由于酸碱的挑战而部分降解。
结论
扫描电子显微镜观察有助于观察胶粘剂对酸碱挑战的反应。修复体周围继发性龋病的预防可能受到修复体和粘结剂材料的物理性质、混合层的质量和氟化物释放的影响。
PURPOSE
To observe the ultrastructure of the dentin-adhesive interface after in vitro sequential challenge by acidic and basic chemicals around adhesive restorations.
MATERIALS AND METHODS
Box-shaped cavities were prepared in bovine root dentin and restored as follows: Reactmer Bond and Reactmer Paste (RB/RP), Clearfil SE Bond and Clearfil AP-X (SE/APX), ABF (experimental) and APX (ABF/APX), Single Bond and APX (SB/APX). After the specimens were stored in water for 1 week, the integrity of the bonds tested by sequential immersion were placed in an artificial demineralizing solution (pH 4.5) for 20 min and in 5% NaOCl for 20 min. The specimens were sectioned, polished, then argon-ion etched for 7 min, and gold sputter coated for SEM examination of the dentin-adhesive interface.
RESULTS
The morphological results indicated tight bonding between the cavity wall dentin and the adhesive. For the fluoride-releasing restoration, RB/RP, a thick acid-resistant zone was clearly observed adjacent to the restoration; however, the bonding resin, RB, was partially degraded by the acid-base challenge. For SE/APX, ABF/APX and SB/APX, the bonding resins were resistant to the acid-base challenge. With the self-etching adhesive systems, SE and ABF, a thin band of acid-base resistant dentin less than 1 microm thick was observed beneath the hybrid layer. With the wet bonding system, SB, the hybrid layer was partially degraded by the acid-base challenge.
CONCLUSION
SEM observation is useful for observing the reactions of adhesives to acid-base challenge. Prevention of secondary caries around a restoration may be influenced by the physical properties of the restorative and adhesive materials, quality of the hybrid layer, and fluoride-release.