Evaluations of Tumor Acidosis Within In Vivo Tumor Models Using Parametric Maps Generated with Acido CEST MRI.
Evaluations of Tumor Acidosis Within In Vivo Tumor Models Using Parametric Maps Generated with Acido CEST MRI.
复制标题
DOI：
10.1007/s11307-014-0816-2
复制
发表时间：
2015-08
影响因子：
3.1
通讯作者：
Pagel MD
中科院分区：
文献类型：
--
作者：
Chen LQ;Randtke EA;Jones KM;Moon BF;Howison CM;Pagel MD
我们的目标是开发肿瘤酸中毒的像素化地图，以帮助评估癌症生物学中的细胞外肿瘤pH(Phe)。在一项纵向研究中，对MCF-7和MDA-MB-231小鼠模型进行了成像。使用AcidoCEST MRI和一系列图像处理方法生成肿瘤Phe参数图，并用pH微型传感器测量肿瘤Phe。通过使用标准的图像处理方法，可以获得足够的对比度和噪声来生成Phe图。将acidoCEST MRI和pH微型传感器测量的Phe值进行比较，结果表明，AidoCEST MRI测量肿瘤Phe的准确度为0.034个pH单位。与MDA-MB-231肿瘤模型相比，MCF-7肿瘤模型的酸性更强。在纵向研究中，Phe与肿瘤大小无关。这些结果表明，acidoCEST MRI可以创建小鼠癌症模型的像素化肿瘤Phe图。
We aimed to develop pixelwise maps of tumor acidosis to aid in evaluating extracellular tumor pH (pHe) in cancer biology. MCF-7 and MDA-MB-231 mouse models were imaged during a longitudinal study. AcidoCEST MRI and a series of image processing methods were used to produce parametric maps of tumor pHe, and tumor pHe was also measured with a pH microsensor. Sufficient contrast-to-noise for producing pHe maps was achieved by using standard image processing methods. A comparison of pHe values measured with acidoCEST MRI and a pH microsensor showed that acidoCEST MRI measured tumor pHe with an accuracy of 0.034 pH units. The MCF-7 tumor model was found to be more acidic compared to the MDA-MB-231 tumor model. The pHe was not related to tumor size during the longitudinal study. These results show that acidoCEST MRI can create pixelwise tumor pHe maps of mouse models of cancer.