Characterization of a SR protein from Trypanosoma brucei with homology to RNA-binding cis-splicing proteins
Characterization of a SR protein from Trypanosoma brucei with homology to RNA-binding cis-splicing proteins
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DOI：
10.1016/s0166-6851(99)00091-2
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发表时间：
1999-07-30
影响因子：
1.5
通讯作者：
Pays, E
中科院分区：
文献类型：
--
作者：
Ismaïli, N;Pérez-Morga, D;Pays, E
布鲁氏锥虫依赖于反式剪接来处理其mRNAs。研究了一种新的富含核丝氨酸/精氨酸(SR)的锥体蛋白(TSR1)，该蛋白含有两个RNA识别基序。TSR1蛋白似乎与高等真核生物的顺式剪接机制的RNA结合SR蛋白同源。此外，在酵母双杂交系统中，TSR1能够与参与识别3‘剪接位点(U2AF35/U2AF65)的人类剪接因子相互作用。在布氏毛滴虫的原循环和血流形式中，都发现TSR1定位于细胞核。在血流阶段，TSR1表现出参与顺式剪接的SR蛋白的斑点模式特征。此外，TSR1能够通过酵母三杂交系统与锥虫体内参与反式剪接的剪接前导(SL)RNA特异性结合。这些和其他观察结果表明，TSR1可能参与了布鲁氏锥虫的性状拼接。(C)1999 Elsevier Science B.V.保留所有权利。
The protozoan parasite Trypanosoma brucei relies on trans-splicing to process its mRNAs. A novel nuclear serine/arginine (SR)-rich trypanosomal protein (TSR1) was characterized which contains two RNA recognition motifs. The TSR1 protein appears to be homologous to RNA-binding SR proteins of the cis-splicing machinery from higher eukaryotes. Moreover, in the yeast two-hybrid system, TSR1 is able to interact with the human splicing factors involved in the recognition of the 3' splicing site (U2AF35/U2AF65). In both procyclic and bloodstream forms of T. brucei, TSR1 was found to localize in the nucleus. In the bloodstream stage TSR1 showed the speckles pattern characteristic of SR proteins involved in cis-splicing. Moreover, TSR1 was able to specifically bind the spliced leader (SL) RNA involved in trans-splicing in trypanosomes by the yeast three-hybrid system. These and other observations suggest that TSR1 may be involved in traits-splicing in T. brucei. (C) 1999 Elsevier Science B.V. All rights reserved.