Detection of SPO11-oligonucleotide complexes from mouse testes.
Detection of SPO11-oligonucleotide complexes from mouse testes.
复制标题
检测小鼠睾丸中的 SPO11-寡核苷酸复合物。
DOI：
10.1007/978-1-59745-527-5_13
复制
发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Keeney,Scott
中科院分区：
文献类型：
--
作者：
Pan,Jing;Keeney,Scott
SPO 11蛋白产生启动减数分裂重组的程序性DNA双链断裂（DSB）。内切核酸裂解3′端至DSB位点，从DNA中释放SPO 11，留下与寡核苷酸共价结合的SPO 11。本章介绍了检测释放产物，SPO 11-寡核苷酸复合物，从小鼠睾丸裂解物。还提供了测定SPO 11相关寡核苷酸大小的方法。
The SPO11 protein generates programmed DNA double-strand breaks (DSBs) that initiate meiotic recombination. Endonucleolytic cleavage 3′ to the DSB sites releases SPO11 from DNA, leaving SPO11 covalently associated with an oligonucleotide. This chapter describes detection of the release product, SPO11-oligonucleotide complexes, from mouse testis lysates. The method for determining the size of SPO11-associated oligonucleotides is also provided.