Small molecule fluorescent voltage indicators for studying membrane potential.
Small molecule fluorescent voltage indicators for studying membrane potential.
复制标题
DOI：
10.1016/j.cbpa.2016.06.003
复制
发表时间：
2016-08
影响因子：
7.8
通讯作者：
Miller EW
中科院分区：
文献类型：
--
作者：
Miller EW
电压成像有可能揭示膜电压的快速变化对细胞生理学的贡献，特别是在神经科学的背景下。特别是，小分子荧光团特别有吸引力，因为理论上它们可以提供膜电位动力学的快速且灵敏的测量。我们将讨论许多类别的小分子电压指示剂，包括具有改进的双光子电压传感功能的染料、用于体内应用的近红外光学轮廓，以及新开发的基于电子转移的指示剂或VoltageFluors，它可以在一系列波长上进行调谐，以实现全光学电压操纵和测量。还将讨论电压指示器的局限性和“愿望清单”。
Voltage imaging has the potential to unravel the contributions that rapid changes in membrane voltage make to cellular physiology, especially in the context of neuroscience. In particular, small molecule fluorophores are especially attractive because they can, in theory, provide fast and sensitive measurements of membrane potential dynamics. A number of classes of small molecule voltage indicators will be discussed, including dyes with improved two-photon voltage sensing, near infrared optical profiles for use in in vivo applications, and newly developed electron-transfer based indicators, or VoltageFluors, that can be tuned across a range of wavelengths to enable all-optical voltage manipulation and measurement. Limitations and a “wish-list” for voltage indicators will also be discussed.