Structural and functional human retinal imaging with a fiber-based visible light OCT ophthalmoscope
Structural and functional human retinal imaging with a fiber-based visible light OCT ophthalmoscope
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DOI：
10.1364/boe.8.000323
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发表时间：
2017-01-01
影响因子：
3.4
通讯作者：
Srinivasan, Vivek J.
中科院分区：
文献类型：
--
作者：
Chong, Shau Poh;Bernucci, Marcel;Srinivasan, Vivek J.
介绍了一种基于光纤的多功能光学相干层析成像(OCT)系统的设计，该系统可用于人体视网膜的轴向分辨率为2微米的组织成像。详细描述了两种不同脉冲重复频率的超连续谱光源的噪声特性。较高的重复频率和较低的噪声源可以使角膜在0.15 mW的光功率和98微秒的曝光时间下获得96分贝的灵敏度。使用专为可见光波长设计的宽带(560+/-50 nm)、90/10、熔融式单模光纤耦合器，样品臂被集成到检眼镜平台中，类似于目前的临床OCT系统。为了演示仪器的操作范围，活体结构视网膜成像还显示了0.15 mW的曝光，每秒10,000和70,000次轴向扫描(后者与商用OCT系统相当)，以及0.03 mW的曝光和每秒10,000次轴向扫描(低于最大允许的连续曝光水平)。最后，还展示了人体视网膜的解剖、饱和度和血红蛋白含量的体内光谱成像。(C)2016年度美国光学学会
The design of a multi-functional fiber-based Optical Coherence Tomography (OCT) system for human retinal imaging with < 2 micron axial resolution in tissue is described. A detailed noise characterization of two supercontinuum light sources with different pulse repetition rates is presented. The higher repetition rate and lower noise source is found to enable a sensitivity of 96 dB with 0.15 mW light power at the cornea and a 98 microsecond exposure time. Using a broadband (560 +/- 50 nm), 90/10, fused single-mode fiber coupler designed for visible wavelengths, the sample arm is integrated into an ophthalmoscope platform, similar to current clinical OCT systems. To demonstrate the instrument's range of operation, in vivo structural retinal imaging is also shown at 0.15 mW exposure with 10,000 and 70,000 axial scans per second (the latter comparable to commercial OCT systems), and at 0.03 mW exposure and 10,000 axial scans per second (below maximum permissible continuous exposure levels). Lastly, in vivo spectroscopic imaging of anatomy, saturation, and hemoglobin content in the human retina is also demonstrated. (C) 2016 Optical Society of America