Polyphenol from Rosaroxburghii Tratt Fruit Ameliorates the Symptoms of Diabetes by Activating the P13K/AKT Insulin Pathway in db/db Mice.
Polyphenol from Rosaroxburghii Tratt Fruit Ameliorates the Symptoms of Diabetes by Activating the P13K/AKT Insulin Pathway in db/db Mice.
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刺梨果实中的多酚通过激活db/db小鼠的PI3K/AKT胰岛素通路改善糖尿病症状。
DOI：
10.3390/foods11050636
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发表时间：
2022-02-22
期刊：
影响因子：
--
通讯作者：
Wang H
中科院分区：
文献类型：
--
作者：
Chen C;Tan S;Ren T;Wang H;Dai X;Wang H
全世界约有4%的人口患有2型糖尿病（T2 DM），现有的治疗糖尿病的降糖药物都有一定的副作用。因此，从植物中提取降血糖成分的研究逐渐成为热点。本研究旨在研究刺梨果实中富含多酚的提取物（RP）及其4种成分（IRP 1-4）对db/db小鼠的降血糖作用。结果表明，RP和IRP 1-4口服给药可显著降低db/db小鼠的摄食量、饮水量、空腹血糖（FBG）和血清胰岛素水平。RP和IRP 1-4组的葡萄糖耐受不良、胰岛素抵抗和氧化应激均得到改善。组织学观察表明RP和IRP 1-4能有效地保护肝脏脂肪的损伤和功能障碍。RP和IRP 1-4还通过增加糖原合成酶激酶3β（GSK 3 β）的磷酸化和降低其表达而增加肝和肌糖原含量。db/db小鼠肝脏中葡萄糖激酶（GCK）、磷酸烯醇式丙酮酸羧化酶（PEPCK）和葡萄糖-6-磷酸酶（G6 PC）的活性及其各自的mRNA表达水平在干预组中同时增加和降低。RP和IRP 1-4可显著增加P13激酶的表达和蛋白激酶B（AKT）的磷酸化。提示RP和IRP 1-4通过激活P13 K/AKT信号通路，调节FOXO 1和p-GSK 3 β蛋白的表达，控制肝细胞新生，改善肝糖原储备胰岛素抵抗，具有良好的降血糖作用。因此，RP和IRP 1-4可作为降血糖功能成分，缓解T2 DM症状。
About 4% of the world’s population has type 2 diabetes mellitus (T2DM), and the available hypoglycemic drugs for treating diabetes have some side effects. Therefore, research on the extraction of hypoglycemic components from plants has gradually become popular. This study aimed to investigate the hypoglycemic effects of polyphenol-rich Rosa roxburghii Tratt extract (RP) isolated from Rosa roxburghii Tratt fruit and of four constituents (IRP 1–4 ) isolated from RP on db/db mice. The results indicated that the oral administration of RP and IRP 1–4 could markedly decrease the food intake, water intake, fasting blood glucose (FBG), and serum insulin levels in the db/db mice. Glucose intolerance, insulin resistance, and oxidative stress were ameliorated in the RP and IRP 1–4 groups. Histopathological observation revealed that RP and IRP 1–4 could effectively protect the liver fat against damage and dysfunction. RP and IRP 1–4 also increased the hepatic and muscle glycogen contents by increasing the phosphorylation and reducing the expression of glycogen synthase kinase 3β (GSK3β). The activities of glucokinase (GCK), phosphoenolpyruvate carboxylase (PEPCK), and glucose-6-phosphatase (G6PC) and their respective mRNA expression levels in the liver of db/db mice were simultaneously increased and decreased in the intervention groups. RP and IRP 1–4 significantly increased the expression of phosphatidylinositol 3-kinase (P13K) and the phosphorylation of protein kinase B (AKT). These results indicate that RP and IRP 1–4 exhibit good hypoglycemic effects by activating the P13K/AKT signaling pathway and regulating the expression of FOXO1 and p-GSK3β proteins, controlling hepatic gluconeogenesis and improving hepatic glycogen storage insulin resistance. Therefore, RP and IRP 1–4 could be utilized as the hypoglycemic functional component to alleviate the symptoms of T2DM.
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DOI：
10.1161/hypertensionaha.117.10546
发表时间：
2018-03
期刊：
Hypertension (Dallas, Tex. : 1979)
影响因子：
--
作者：
Tsimihodimos V;Gonzalez-Villalpando C;Meigs JB;Ferrannini E
通讯作者：
Ferrannini E
影响因子：
5.9
作者：
Williamson G;Sheedy K
通讯作者：
Sheedy K
影响因子：
5.6
作者：
Eseberri I;Laurens C;Miranda J;Louche K;Lasa A;Moro C;Portillo MP
通讯作者：
Portillo MP
影响因子：
5.1
作者：
Chen, Ying;Cheng, Feng-Bo;Qin, Nan
通讯作者：
Qin, Nan
影响因子：
7.2
作者：
Moharram, FA;Marzouk, MS;Aboutabl, EA
通讯作者：
Aboutabl, EA