Neuropeptide W is present in antral G cells of rat, mouse, and human stomach.
Neuropeptide W is present in antral G cells of rat, mouse, and human stomach.
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DOI：
10.1677/joe.1.06195
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发表时间：
2006
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影响因子：
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通讯作者：
M. S. Mondal;H. Yamaguchi;Y. Date;K. Toshinai;Takashi Kawagoe;Tomoko Tsuruta;H. Kageyama;Y. Kawamura;Seiji Shioda;Yukio Shimomura;Masatomo Mori;M. Nakazato
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文献类型：
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作者：
M. S. Mondal;H. Yamaguchi;Y. Date;K. Toshinai;Takashi Kawagoe;Tomoko Tsuruta;H. Kageyama;Y. Kawamura;Seiji Shioda;Yukio Shimomura;Masatomo Mori;M. Nakazato
神经肽W（NPW）是一种30个氨基酸的肽，最初从猪下丘脑中分离出来，作为G蛋白偶联受体GPR 7和GPR 8的内源性配体。侧脑室注射NPW可增加血清催乳素和皮质酮浓度，减少暗相摄食，提高能量消耗，降低体重。在外周，GPR 7受体在整个胃肠道中大量表达;然而，NPW在胃肠道内分泌系统中的存在尚未研究。使用单克隆和多克隆抗体对大鼠NPW，我们研究了NPW在大鼠，小鼠和人胃的光镜和电镜定位。在所有三个物种的胃窦腺内均发现了NPW免疫反应细胞。免疫组化和电镜免疫组化双重研究表明，NPW存在于胃窦胃泌素（G）细胞。NPW免疫反应定位于圆形，中到高密度颗粒在G细胞。在大鼠胃窦腺中，NPW免疫反应细胞占90%的染色质A免疫反应细胞和85%的胃泌素免疫反应细胞。使用反相HPLC结合NPW特异性酶免疫测定，我们在胃粘膜中检测到NPW 30及其C端截短形式NPW 23。胃窦血浆NPW浓度显著高于体静脉，提示循环NPW来源于胃。禁食15 h后胃窦血浆NPW浓度显著降低，再进食后显著升高。这是第一份阐明大鼠、小鼠和人类胃中存在NPW肽的报告。总之，NPW产生于胃窦G细胞;我们的研究结果将为这种新型脑/肠肽调节胃功能的其他机制提供线索。
Neuropeptide W (NPW) is a 30-amino-acid peptide initially isolated from the porcine hypothalamus as an endogenous ligand for the G protein-coupled receptors GPR7 and GPR8. An intracerebroventricular administration of NPW increased serum prolactin and corticosterone concentrations, decreased dark-phase feeding, raised energy expenditure, and lowered body weight. Peripherally, GPR7 receptors are abundantly expressed throughout the gastrointestinal tract; the presence of NPW in the gastrointestinal endocrine system, however, remains unstudied. Using monoclonal and polyclonal antibodies raised against rat NPW, we studied the localization of NPW in the rat, mouse, and human stomach by light and electron microscopy. NPW-immunoreactive cells were identified within the gastric antral glands in all three species. Double immunohistochemistry and electron-microscopic immunohistochemistry studies in rats demonstrated that NPW is present in antral gastrin (G) cells. NPW immunoreactivity localized to round, intermediate-to-high-density granules in G cells. NPW-immunoreactive cells accounted for 90% chromagranin A- and 85% gastrin-immunoreactive endocrine cells in the rat gastric antral glands. Using reversed-phase HPLC coupled with enzyme immunoassays specific for NPW, we detected NPW30 and its C-terminally truncated form, NPW23, in the gastric mucosa. Plasma NPW concentration of the gastric antrum was significantly higher than that of the systemic vein, suggesting that circulating NPW is derived from the stomach. Plasma NPW concentration of the gastric antrum decreased significantly after 15-h fast and increased after refeeding. This is the first report to clarify the presence of NPW peptide in the stomachs of rats, mice, and humans. In conclusion, NPW is produced in gastric antral G cells; our findings will provide clues to additional mechanisms of the regulation of gastric function by this novel brain/gut peptide.