Expression and activity of the Fas antigen in bovine ovarian follicle cells.
Expression and activity of the Fas antigen in bovine ovarian follicle cells.
复制标题
Fas 抗原在牛卵巢卵泡细胞中的表达和活性。
DOI：
10.1095/biolreprod62.1.54
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发表时间：
2000
影响因子：
3.6
通讯作者：
Quirk,SM
中科院分区：
文献类型：
--
作者：
Vickers,SL;Cowan,RG;Harman,RM;Porter,DA;Quirk,SM
Fas抗原是一种细胞表面受体，当与Fas配体(FasL)结合时会触发细胞凋亡。为了确定奶牛是否提供了一个合适的模型来研究Fas通路在卵泡闭锁过程中诱导卵巢细胞凋亡的作用。检测Fas抗原的表达及对FasL诱导的体外杀伤的反应性。细胞因子肿瘤坏死因子-α和干扰素-γ的作用已被研究，因为它们在Fas介导的其他类型的细胞凋亡中发挥了作用。在培养的颗粒细胞和卵泡膜细胞中均可检测到Fas抗原的表达，经干扰素处理后，Fas抗原的表达明显增加，而对肿瘤坏死因子的作用不明显。颗粒细胞和卵泡膜细胞对FasL诱导的杀伤具有抵抗力，除非预先用干扰素处理。肿瘤坏死因子对FasL诱导的杀伤无影响。对FasL反应产生杀伤作用的颗粒和膜细胞培养物Annexin V染色呈阳性，这是细胞发生凋亡的早期标志。这些结果为利用牛卵巢进一步研究Fas抗原在卵泡闭锁中的作用奠定了基础。
The Fas antigen is a cell surface receptor that triggers apoptosis when bound to Fas ligand (FasL). Studies were undertaken to determine whether the cow provides a suitable model to study the role of the Fas pathway in inducing apoptosis of ovarian cells during follicular atresia. Expression of Fas antigen mRNA and responsiveness to FasL-induced killing in vitro were measured. Effects of the cytokines tumor necrosis factor (TNF)-α and interferon-γ (IFN) were studied because of previous demonstrations of their role in Fas-mediated apoptosis in other cell types. Fas antigen mRNA was detectable in cultured granulosa and theca cells, and expression was increased by treatment with IFN but not TNF. Granulosa and theca cells were resistant to FasL-induced killing unless pretreated with IFN. TNF had no effect on FasL-induced killing. Granulosa and theca cell cultures in which killing occurred in response to FasL stained positively for annexin V, an early marker for cells undergoing apoptosis. These results provide a basis for further studies using the bovine ovary to examine the role of the Fas antigen in follicular atresia.