Plasmonic-based impedance microspectroscopy of optically heterogeneous samples.
Plasmonic-based impedance microspectroscopy of optically heterogeneous samples.
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DOI：
10.1364/boe.395474
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发表时间：
2020-11-01
影响因子：
3.4
通讯作者：
Abayzeed SA
中科院分区：
文献类型：
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作者：
Abayzeed SA
提出了一种稳健的阻抗显微镜技术。这种光学工具能够对电学特性进行高分辨率成像，具有很好的生物物理应用前景。其基本原理是，表面等离子体共振(SPR)传感器能够测量表面电荷密度的扰动，因此可以用来计算表面附着细胞的阻抗。然而，执行可靠的定量阻抗成像的能力受到细胞-传感器界面的光学异质性的影响。为了解决这一问题，提出了一种新的定量时间分辨谐振角跟踪方法，并将其应用于校正光学性质的影响。为了证明这项技术的能力，进行了牛血清白蛋白(BSA)模式的阻抗显微光谱分析，以实现亚微观分辨率的电容测量。这项工作提供了一种阻抗显微光谱学方法，它将为研究单细胞和生物分子的电学性质以及生物电流开辟新的途径。
A robust impedance microscopy technique is presented. This optical tool enables high resolution imaging of electrical properties with promising biophysical applications. The underlying principle is that surface plasmon resonance (SPR) sensors are able to measure perturbations of surface charge density and therefore can be used to compute the impedance of surface-adhered cells. However, the ability to perform reliable quantitative impedance imaging is affected by the optical heterogeneity of the cell-sensor interface. To address this issue, a novel method for quantitative time-resolved resonance angle tracking is developed and applied to correct for the effect of the optical properties. To demonstrate the capability of this technique, impedance microspectroscopy of bovine serum albumin (BSA) patterns was performed enabling measurements of capacitance with submicroscopic resolution. The work presented offers an impedance microspectroscopy method that will create new avenues in studying the electrical properties of single cells and biomolecules as well as bio-electrical currents.