THE EFFECT OF CHEMICAL PRETREATMENT ON THE AEROBIC MICROBIAL-DEGRADATION OF PCB CONGENERS IN AQUEOUS SYSTEMS
THE EFFECT OF CHEMICAL PRETREATMENT ON THE AEROBIC MICROBIAL-DEGRADATION OF PCB CONGENERS IN AQUEOUS SYSTEMS
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DOI：
10.1007/bf01570014
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发表时间：
1995-07-01
期刊：
影响因子：
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通讯作者：
RICE, LE
中科院分区：
文献类型：
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作者：
ARONSTEIN, BN;PATEREK, JR;RICE, LE
通过一系列实验研究了化学预处理对C-14标记的多氯联苯同系物在水溶液中生物降解的影响。采用芬顿试剂产生羟基自由基（OH.）它们成功地部分氧化/转化了四氯化PCB中的难降解分子，但对一氯化同系物的生物降解影响很小或没有影响，芬顿试剂的应用（1%H2O2，1 mM FeSO 4），然后接种纯培养物假单胞菌属（Pseudomonassp）、菌株LB 400和真养产碱菌，菌株H850导致以（CO2）-C-14的形式去除约38%的2-氯联苯和51%的2，2 '，4，4'-四氯联苯。比较应用芬顿试剂后2，2 '，4，4'-四氯联苯的生物降解速率和程度与实验系统水相中放射性的动态和最终水平，表明微生物利用化学氧化改变的四氯PCB同系物的两种可能方法：（a）溶解在水相中的部分氧化的化学品的消耗，和（B）对转化的化合物的直接微生物攻击，所述转化的化合物可能仍然粘附在固体表面上。
A series of experiments was conducted to examine the effects of chemical pretreatment on biodegradation of C-14-labeled PCB congeners in aqueous systems. Fenton's reagent was used to generate hydroxyl radicals (OH.) which were successful in partially oxidizing/transforming otherwise recalcitrant molecules of tetrachlorinated PCB, but had little or no impact on the biodegradation of a monochlorinated congener, Application of Fenton's reagent (1% H2O2, 1 mM FeSO4) followed by inoculation with pure cultures Pseudomonas sp, strain LB 400 and Alcaligenes eutrophus, strain H850 resulted in the removal of approximately 38% of 2-chlorobiphenyl and 51% of 2,2',4,4'-tetrachlorobiphenyl in the form of (CO2)-C-14. Comparison of the rate and extent of biodegradation of 2,2',4,4'-tetrachlorobiphenyl after the application of Fenton's reagent with the dynamic and final level of radioactivity in the aqueous phase of experimental system suggests two possible means of microbial utilization of tetrachlorinated PCB congener altered by chemical oxidation: (a) consumption of the partially oxidized chemical dissolved in the aqueous phase, and (b) direct microbial attack on the transformed compound, which may still be adhered to the solid surface.