Methylation sensitive-sequence related amplified polymorphism (MS-SRAP) marker system and its application to de novo methylation detection in Brassica napus
Methylation sensitive-sequence related amplified polymorphism (MS-SRAP) marker system and its application to de novo methylation detection in Brassica napus
复制标题
甲基化敏感序列相关扩增多态性（MS-SRAP）标记系统及其在甘蓝型油菜甲基化检测中的应用
DOI：
10.5897/ajb10.788
复制
发表时间：
2011-04
影响因子：
--
通讯作者：
Li, Maoteng
中科院分区：
文献类型：
--
作者：
Zhang, Chunyu;Xiao, Yong;Li, Lingjuan;Long, Yan;Li, Maoteng
DNA甲基化是一种重要的表观遗传过程，参与染色质结构的组织，在基因表达调控中起重要作用。序列相关扩增多态性（Sequence-related amplified polymorphism，SRAP）是一种简单而有效的基因扩增标记系统，可用于植物基因定位和基因标签。利用甲基化敏感的限制性内切酶消化基因组DNA，结合SRAP和甲基化敏感的序列相关扩增多态性（MSSRAP）标记系统，研究植物基因组DNA的从头甲基化。为了研究该标记系统的有效性，对甘蓝型油菜W10（DH系）3个个体的5个不同组织的DNA样品进行了从头DNA甲基化分析。结果表明，约0.2%的重复性多态性片段之间的五个不同的组织中发现。测序结果表明，部分多态性片段与GenBank中的叶绿体编码基因、光合作用相关基因和功能未知蛋白编码基因相匹配。因此，MS-SRAP标记系统是一种简单而有效的检测基因从头甲基化的系统。
DNA methylation is one of the most important epigenetic processes that participates in the organization of chromatin structure and plays an important role in regulating gene expression. Sequence-related amplified polymorphism (SRAP) is a simple but an efficient gene amplification marker system for both mapping and gene tagging in plants. Combined methylation sensitive restriction enzyme digested genomic DNA with SRAP and methylation sensitive-sequence related amplified polymorphism (MSSRAP) marker system was developed to investigate de novo DNA methylation in the plant genome. To investigate the efficiency of this new marker system, DNA samples of 5 different tissues from 3 individuals of Brassica napus W10 (DH line) were employed for de novo DNA methylation analysis. Results indicated that approximately 0.2% reproducible polymorphic fragments were found among the five different tissues. Sequencing results demonstrated that some of these polymorphic fragments were matched well with the chloroplast encoding genes, photosynthetic related genes and the genes encoding protein with unknown functions in Genbank. Therefore, MS-SRAP marker system is a simple but efficient system for detecting gene de novo methylation.
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影响因子：
2.1
作者：
Peggy Horn;A. Rafalski
通讯作者：
Peggy Horn;A. Rafalski
影响因子：
11.2
作者：
Huidong Shi;Susan H. Wei;Yu‐Wei Leu;F. Rahmatpanah;Joseph C. Liu;P. Yan;K. Nephew;T. Huang
通讯作者：
Huidong Shi;Susan H. Wei;Yu‐Wei Leu;F. Rahmatpanah;Joseph C. Liu;P. Yan;K. Nephew;T. Huang
DOI：
10.1073/pnas.93.16.8334
发表时间：
1996-08-06
影响因子：
11.1
作者：
Hohn, T;Corsten, S;Rothnie, H
通讯作者：
Rothnie, H
影响因子：
5.4
作者：
Li, G;Quiros, CF
通讯作者：
Quiros, CF
影响因子：
20.3
作者：
Singal, R;vanWert, JM
通讯作者：
vanWert, JM