Definition of the mycobacterial SOS box and use to identify LexA-regulated genes in Mycobacterium tuberculosis
Definition of the mycobacterial SOS box and use to identify LexA-regulated genes in Mycobacterium tuberculosis
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DOI：
10.1128/jb.184.12.3287-3295.2002
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发表时间：
2002-06-01
影响因子：
3.2
通讯作者：
Rand, L
中科院分区：
文献类型：
--
作者：
Davis, EO;Dullaghan, EM;Rand, L
分枝杆菌SOS盒的碱基对莱克萨结合很重要，通过用每个碱基替换每个碱基并检查DNA损伤后对报告基因诱导的影响来确定。该分析显示，SOS盒在回文位点的每一半中比最初认为的长2bp。用新的共有序列TCGAAC（N）（4）GTT CGA搜索结核分枝杆菌基因组序列，鉴定出4个完全匹配的位点和12个预测与莱克萨结合的具有单一错配的位点。可能被这些SOS盒调控的基因从它们相对于位点的位置确定。对DNA损伤后这些基因的表达数据的检查鉴定了12个新的基因，它们最有可能受莱克萨以及已知的M.结核病DNA损伤诱导基因recA、莱克萨和ruvC。在这12个基因中，只有2个具有预测的功能：DNA聚合酶III的组分dnaE 2和linB，其类似于1，3，4，6-四氯-1，4-环己烷二烯水解酶。奇怪的是，在预测受莱克萨调控的其余10个基因中，有7个是W.结核病13 E12重复序列家族，其中具有部分移动的特征的元件。
The bases of the mycobacterial SOS box important for LexA binding were determined by replacing each base with every other and examining the effect on the induction of a reporter gene following DNA damage. This analysis revealed that the SOS box was longer than originally thought by 2 bp in each half of the palindromic site. A search of the Mycobacterium tuberculosis genome sequence with the new consensus, TCGAAC(N)(4)GTT CGA, identified 4 sites which were perfect matches and 12 sites with a single mismatch which were predicted to bind LexA. Genes which could potentially be regulated by these SOS boxes were ascertained from their positions relative to the sites. Examination of expression data for these genes following DNA damage identified 12 new genes which are most likely regulated by, LexA as well as the known M. tuberculosis DNA damage-inducible genes recA, lexA, and ruvC. Of these 12 genes, only 2 have a predicted function: dnaE2, a component of DNA polymerase III, and linB, which is similar to 1,3,4,6-tetrachloro-1,4-cylcohexadiene hydrolase. Curiously, of the remaining 10 genes predicted to be LexA regulated, 7 are members of the W. tuberculosis 13E12 repeat family, which has some of the characteristics of mobile elements.