REVEALING CRYPTIC PARASITE DIVERSITY IN A DEFINITIVE HOST: ECHINOSTOMES IN MUSKRATS
REVEALING CRYPTIC PARASITE DIVERSITY IN A DEFINITIVE HOST: ECHINOSTOMES IN MUSKRATS
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DOI：
10.1645/ge-3117.1
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发表时间：
2012-12-01
影响因子：
1.3
通讯作者：
Belden, Lisa K.
中科院分区：
文献类型：
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作者：
Detwiler, Jillian T.;Zajac, Anne M.;Belden, Lisa K.
许多吸虫类群有很长的系统修订历史，这使得寄生虫鉴定成为一项艰巨的任务。麝鼠的吸虫寄生虫也不例外。在这里，我们强调与麝鼠吸虫(Ondatra Zibethicus)的系统性问题。然后，我们演示了使用形态和分子工具来识别这些寄生虫的效用。首次对63只麝鼠标本进行了形态观察，发现至少有4个属，包括棘口吸虫属、华迪氏吸虫属、Quinquerialis属和Notocoylus属。对于后3组，28S区域验证了这一评估。对于甲壳类，ND1序列至少揭示了5个遗传谱系。一个特殊的血统，三刺棘口吸虫血统b，在感染率和感染强度上都占主导地位。分子序列提供了对麝鼠棘口动物多样性的更准确的估计，并进一步支持了三刺棘口棘口动物是一个物种复合体的观点。未来的研究将集中在三伏埃希氏菌谱系之间是否存在寄主特异性的差异。此外，这项研究还提供了初步的序列，这将有助于验证Wardius，Quinquerialis，特别是Notocoylus的生活史。通过将分子、形态和生活史信息联系起来，我们可以更好地了解寄生虫的多样性。
Many trematode groups have a long history of systematic revision, which can make parasite identification a difficult task. The trematode parasites of muskrats are no exception. Here, we highlight the systematic issues associated with trematodes of muskrats (Ondatra zibethicus). Then, we demonstrate the utility of using both morphological and molecular tools to identify these parasites. Morphological examinations of specimens from muskrats (n = 63) first suggested that at least 4 genera were present including Echinostoma, Wardius, Quinqueserialis, and Notocotylus. For the latter 3 groups, the 28S region verified this assessment. For echibostomes, ND1 sequences revealed at least 5 genetic lineages. A particular lineage, Echinostoma trivolvis lineage b, predominated in both prevalence and intensity of infection. Molecular sequences provided a more accurate estimate of echinostome diversity in the muskrats and further support the idea that E. trivolvis is a species complex. Future studies will focus on whether there are differences in host specificity among the E. trivolvis lineages. In addition, this study has provided initial sequences that will help verify the life cycles of Wardius, Quinqueserialis, and especially, Notocotylus. By linking molecular, morphological, and life history information, we can better understand parasite diversity.