Rickettsia, Ehrlichia, Anaplasma, and Bartonella in Ticks and Fleas from Dogs and Cats in Bangkok

Rickettsia, Ehrlichia, Anaplasma, and Bartonella in Ticks and Fleas from Dogs and Cats in Bangkok
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DOI:
10.1089/vbz.2010.0174
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发表时间:
2011-10-01
影响因子:
2.1
通讯作者:
Gaywee, Jariyanart
Gaywee, Jariyanart
中科院分区:
医学4区
文献类型:
--
作者:
Foongladda, Suporn;Inthawong, Dutsadee;Gaywee, Jariyanart

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用聚合酶链式反应和DNA测序技术对曼谷不同地点的猫、狗的蚤类标本(5-10只)进行检测,以检测立克次体(GLTA和17 kDa基因)、无浆体科(16S rRNA基因)和巴尔通体(Pap31及其基因)的DNA。经鉴定,该菌为立克次体。在98只犬蚤标本中检出66只(67.4%)与立克次体有关的鼠疫杆菌,在54只猫蚤标本中检出10只(18.5%)亨氏巴尔通体和2只克氏巴尔通体。此外,这项工作首次提供了10只犬埃立克体(3.3%)、7只无浆体(2.3%)和2株沃尔巴克氏杆菌的证据。泰国304份血吸虫标本中检出阳性率为0.66%。
Flea and tick specimens (5-10 fleas or ticks) on dogs and cats from various sites in Bangkok were tested by polymerase chain reaction and DNA sequencing to detect DNA of bacteria Rickettsia (gltA and 17 kDa genes), Anaplasmataceae (16S rRNA gene), and Bartonella (pap31 and its genes). We confirmed that Rickettsia sp. related to Rickettsia felis was detected in 66 of 98 (67.4%) flea specimens from dogs, whereas 8 Bartonella henselae and 2 Bartonella clarridgeiae were detected in 10 of 54 (18.5%) flea specimens from cats. Further, this work provides the first evidence of 10 Ehrlichia canis (3.3%), 7 Anaplasma platys (2.3%), and 2 Wolbachia spp. (0.66%) in 304 Rhipicephalus sanguineus tick specimens in Thailand.