Cartography of Hematopoietic Stem Cell Commitment Dependent upon a Reporter for Transcription Factor Activation
Cartography of Hematopoietic Stem Cell Commitment Dependent upon a Reporter for Transcription Factor Activation
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DOI：
10.1196/annals.1392.009
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发表时间：
2007-06
影响因子：
5.2
通讯作者：
K. Akashi
中科院分区：
文献类型：
--
作者：
K. Akashi
摘要：基于谱系限制性祖细胞的前瞻性纯化结果，提出了一种分层造血发育树。为了更详细地定位造血干细胞（HSC）定型，我们通过使用携带PU.1敲入报告基因的小鼠系，从HSC到髓淋巴样祖细胞阶段跟踪了PU.1（一种主要的粒细胞/单核细胞（GM）和淋巴相关转录因子）的表达。这种方法使我们能够找到一个新的祖细胞群体致力于GM和HSC部分内的淋巴谱系。这一结果表明，应该有另一个独立于传统的发展途径与髓与淋巴分叉，共同的髓祖细胞和共同的淋巴祖细胞，分别代表。利用转录因子表达作为功能标记可能有助于获得更高分辨率的造血发育图。
Abstract: A hierarchical hematopoietic developmental tree has been proposed based on the result of prospective purification of lineage‐restricted progenitors. For more detailed mapping for hematopoietic stem cell (HSC) commitment, we tracked the expression of PU.1, a major granulocyte/monocyte (GM)‐ and lymphoid‐related transcription factor, from the HSC to the myelolymphoid progenitor stages by using a mouse line harboring a knockin reporter for PU.1. This approach enabled us to find a new progenitor population committed to GM and lymphoid lineages within the HSC fraction. This result suggests that there should be another developmental pathway independent of the conventional one with myeloid versus lymphoid bifurcation, represented by common myeloid progenitors and common lymphoid progenitors, respectively. The utilization of the transcription factor expression as a functional marker might be useful to obtain cartography of the hematopoietic development at a higher resolution.