The effects of dopamine on temperature regulation in goldfish.
The effects of dopamine on temperature regulation in goldfish.
复制标题
多巴胺对金鱼温度调节的影响。
DOI：
10.1007/bf00692686
复制
发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Panayiotides-Djaferis,H
中科院分区：
文献类型：
--
作者：
Wollmuth,LP;Crawshaw,LI;Panayiotides-Djaferis,H
在鲫鱼（Carassius auratus: 40-85 g）的特定前脑位点注射多巴胺（DA），研究DA在行为体温调节中的作用。在视前脑室周围核（NPP）的前部注射25、50、100和250 ng DA，在注射5或10 ng DA后，观察到选择温度一致的剂量依赖性降低。注射对照液无效果。将DA注射到其他前脑位点，包括NPP的后半部分，要么没有热调节作用，要么有轻微的热调节作用，与注射到最有效的部位相比，这是不一致的，需要更大的剂量才能获得。在NPP前部注射100 ng DA后，选择温度的下降被氟哌啶醇（一种多巴胺能拮抗剂）阻断，但不被酚妥拉明（一种去肾上腺素能拮抗剂）阻断。单独注射氟哌啶醇导致选择温度轻微但有统计学意义的升高。最敏感的DA部位位于NPP前部对去甲肾上腺素最敏感的部位的尾侧。DA作用于金鱼NPP前部中枢热调节神经元的多巴胺能受体。这些受体似乎介导了对过度温暖环境的行为反应。
Microinjections of dopamine (DA) were made into specific forebrain loci in goldfish (Carassius auratus: 40–85 g) to study the involvement of DA in behavioral thermoregulation. Injections of 25, 50, 100 and 250 ng DA into the anterior aspect of the nucleus preopticus periventricularis (NPP) led to consistent, dose-dependent decreases in selected temperature was observed following injections of 5 or 10 ng DA. Injections of the control solution were without effect.Injections of DA into other forebrain loci, including the posterior half of the NPP, either had no thermoregulatory effect or had minor thermoregulatory effects which, in comparison to injections into the most effective sites, were inconsistent and required larger doses to obtain. The decrease in selected temperature following injections of 100 ng DA into the anterior NPP was blocked by haloperidol, a dopaminergic antagonist, but not by phentolamine, a noradrenergic antagonist. Injections of haloperidol alone resulted in a minor, but statistically significant, increase in selected temperature.The most sensitive DA sites lie caudal to the sites most sensitive to norepinephrine within the anterior NPP. DA acts on the dopaminergic receptors of central thermoregulatory neurons in the anterior NPP of goldfish. These receptors appear to mediate behavioral responses to excessively warm environments.