Temporal changes and the effect of subtherapeutic concentrations of antibiotics in the gut microbiota of swine
Temporal changes and the effect of subtherapeutic concentrations of antibiotics in the gut microbiota of swine
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DOI：
10.1111/1574-6941.12419
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发表时间：
2014-12-01
影响因子：
4.2
通讯作者：
Chenier, Martin R.
中科院分区：
文献类型：
--
作者：
Holman, Devin B.;Chenier, Martin R.
为了促进猪的生长，在养猪生产中使用抗生素可以追溯到20世纪50年代。尽管有这么长的使用历史，但抗生素对猪生长促进作用的确切机制在很大程度上仍然未知。然而，人们认为，促进生长是由于抗生素对肠道微生物群有直接影响。在本研究中，采用illumina测序技术，研究了两种抗生素对猪肠道微生物群的影响，为期19周。在饲喂亚治疗浓度泰乐素（4411 mg kg（-1）饲料）的猪中，观察到几个分类群和26个操作分类单位（otu）的相对丰度发生了变化。在5.5 mg kg（-1）饲料中给予金四素时，只观察到轻微的变化。在哺乳仔猪和断奶仔猪之间，分类群和OTUs的相对丰度变化最为显著。利用Shannon、Chao1和系统发育多样性指数测量，哺乳仔猪肠道微生物群的多样性也有所降低。这些结果表明，抗生素对猪肠道微生物群的影响是根据剂量和持续时间而变化的，并且猪肠道微生物群对抗生素扰动引起的长期变化表现出相当大的弹性。
The use of antibiotics in swine production for the purpose of growth promotion dates back to the 1950s. Despite this long history of use, the exact mechanism(s) responsible for the growth-promoting effects of antibiotics in swine remain largely unknown. It is believed, however, that growth promotion is due to antibiotics having a direct impact on the gut microbiota. In this study, the effect of two antibiotics on the swine gut microbiota over a 19-week monitoring period was investigated using Illumina-based sequencing. A shift in the relative abundance of several taxa and in 26 operational taxonomic units (OTUs) was observed in pigs fed subtherapeutic concentrations of tylosin (4411 mg kg(-1) feed). Only minor alterations were noted with the administration of chlortetracycline at 5.5 mg kg(-1) feed. The most notable changes in the relative abundance of taxa and OTUs were noted between suckling piglets and postweaned pigs. Diversity was also reduced in the gut microbiota of suckling piglets as measured using the Shannon, Chao1, and phylogenetic diversity indices. These results show that the effect of antibiotics on the swine gut microbiota is variable based on dosage and duration and that the swine gut microbiota exhibits considerable resilience to long-term changes due to antibiotic perturbations.