Production and characterization of somatic hybrids between Arabidopsis thaliana and Brassica nigra
Production and characterization of somatic hybrids between Arabidopsis thaliana and Brassica nigra
复制标题
拟南芥和黑芥子体细胞杂种的生产和表征
DOI：
10.1016/0168-9452(95)04221-f
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发表时间：
1995
期刊：
影响因子：
5.2
通讯作者：
M. Sacristán
中科院分区：
文献类型：
--
作者：
J. Siemens;M. Sacristán
用完整的芥菜原生质体与辐照过的芥菜原生质体融合，获得了十字花科植物A.thaliana和B.ngra的体细胞杂种。以黑曲霉对潮霉素的抗性为融合产物的筛选标准，可筛选出66个愈伤组织系。根据聚乙二醇法处理的拟南芥原生质体总数，推定的融合频率为1.5×10−8，这一频率大大低于采用相同方法通过两个近等基因系之间的融合而获得的融合频率。三个杂交愈伤组织系永久再生不定芽。通过聚合酶链式反应和Southern分析，证明了两个形态中等的杂交品系的近对称性，以及一个形态为黑麦草的品系的高度不对称性。
Somatic hybrids between the Brassicaceae species A. thaliana and B. nigra have been produced by fusion of intact A. thaliana protoplasts with irradiated B. nigra protoplasts. Sixty-six callus lines could be selected applying the hygromycin resistance present in B. nigra as selection criterion for fusion products. In regard to the total number of PEG-treated A. thaliana protoplasts, this corresponded to a putative fusion frequency of 1.5 × 10−8, a frequency considerably lower than such obtained, applying the same protocol, by a fusion between two nearly isogenic lines of A. thaliana. Three hybrid callus lines permanently regenerated shoots. By PCR- and Southern-analysis the nearly symmetric character of two hybrid lines with an intermediate morphology, and the highly asymmetric character of one line with a B. nigra morphology could be proven.