Evolutionary changes in germ granule mRNA content are driven by multiple mechanisms in Drosophila.
Evolutionary changes in germ granule mRNA content are driven by multiple mechanisms in Drosophila.
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果蝇中胚芽颗粒 mRNA 含量的进化变化是由多种机制驱动的。
DOI：
10.1101/2023.02.21.529147
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Niepielko,MatthewG
中科院分区：
文献类型：
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作者：
Doyle,DominiqueA;Burian,FlorenciaN;Aharoni,Benjamin;Klinder,AnnabelleJ;Menzel,MelissaM;Nifras,GerardCarloC;Shabazz-Henry,AhadL;Palma,BiancaUlrich;Hidalgo,GisselleA;Sottolano,ChristopherJ;Ortega,BiancaM;Niepielko,MatthewG
将 mRNA 共同包装成称为胚芽颗粒的生物分子凝聚物，是转录后调节在种系发育和维持中发挥作用的 mRNA 的保守策略。在黑腹果蝇中，mRNA 通过形成同型簇（包含来自特定基因的多个转录本的聚集体）在胚芽颗粒中积累。由 Oskar (Osk) 成核，黑腹果蝇中的同型簇是通过随机播种和自我招募过程生成的，该过程需要胚芽颗粒 mRNA 的 3' UTR。有趣的是，属于胚芽颗粒 mRNA（例如 nanos (nos)）的 3' UTR 在果蝇物种之间具有相当大的序列变异。因此，我们假设 3' UTR 的进化变化影响胚芽颗粒的发育。为了检验我们的假设，我们研究了四种果蝇物种中 nos 和极性颗粒成分 (pgc) 的同型聚类，并得出结论：同型聚类是用于富集胚芽颗粒 mRNA 的保守发育过程。此外，我们发现在 nos 和/或 pgc 簇中发现的转录本数量在不同物种之间可能存在显着差异。通过将生物数据与计算模型相结合，我们确定了自然发生的胚芽颗粒多样性的多种机制，包括 nos、pgc、osk 水平和/或同型聚类功效的变化。最后，我们发现来自不同物种的nos 3'UTR可以改变nos同型聚类的功效，从而导致胚芽颗粒的nos积累减少。我们的研究结果强调了进化对胚芽颗粒发育的影响，并可能提供对改变其他类别生物分子凝聚物含量的过程的见解。
The co-packaging of mRNAs into biomolecular condensates called germ granules is a conserved strategy to post-transcriptionally regulate mRNAs that function in germline development and maintenance. In D. melanogaster, mRNAs accumulate in germ granules by forming homotypic clusters, aggregates that contain multiple transcripts from a specific gene. Nucleated by Oskar (Osk), homotypic clusters in D. melanogaster are generated through a stochastic seeding and self-recruitment process that requires the 3′ UTR of germ granule mRNAs. Interestingly, the 3′ UTR belonging to germ granule mRNAs, such as nanos (nos), have considerable sequence variations among Drosophila species. Thus, we hypothesized that evolutionary changes in the 3′ UTR influences germ granule development. To test our hypothesis, we investigated the homotypic clustering of nos and polar granule component (pgc) in four Drosophila species and concluded that homotypic clustering is a conserved developmental process used to enrich germ granule mRNAs. Additionally, we discovered that the number of transcripts found in nos and/or pgc clusters could vary significantly among species. By integrating biological data with computational modeling, we determined that multiple mechanisms underlie naturally occurring germ granule diversity, including changes in nos, pgc, osk levels, and/or homotypic clustering efficacy. Finally, we found that the nos 3′ UTR from different species can alter the efficacy of nos homotypic clustering, resulting in germ granules with reduced nos accumulation. Our findings highlight the impact that evolution has on the development of germ granules and may provide insight into processes that modify the content of other classes of biomolecular condensates.