Temporal, 3-dimensional, cellular anatomy of corneal wound tissue.
Temporal, 3-dimensional, cellular anatomy of corneal wound tissue.
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DOI：
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发表时间：
1995-04
影响因子：
2.4
通讯作者：
James V. Jester;W. Petroll;P. Barry;H. Cavanagh
中科院分区：
文献类型：
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作者：
James V. Jester;W. Petroll;P. Barry;H. Cavanagh
我们已经评估了时间的三维细胞解剖的角膜伤口组织在兔眼在体内串联扫描共聚焦显微镜。体内显微镜研究表明，角膜成纤维细胞迁移到伤口作为一个相互连接的细胞网络与长，薄，随机取向的细胞过程。通过连接蛋白43的单克隆抗体的定位进一步证实了成纤维细胞的相互连接，连接蛋白43显示出成纤维细胞之间推定的间隙连接的显著染色。时间的观察表明，相互连接的细胞和细胞过程经历顺序的位置变化，导致方向的细胞和相互连接的细胞过程平行于伤口边缘。激光扫描共聚焦显微镜的整块，鬼笔环肽染色的角膜伤口显示突出的细胞内f-肌动蛋白束（即应力纤维）的细胞过程中，形成了广泛的交织图案内的伤口。
We have evaluated temporally the 3-dimensional cellular anatomy of corneal wound tissue in the rabbit eye using in vivo tandem scanning confocal microscopy. In vivo microscopic studies showed that corneal fibroblast migrated into the wound as an interconnected cellular meshwork with long, thin, randomly oriented cell processes. Interconnection of fibroblasts was further confirmed by localisation of monoclonal antibodies to connexin 43 which demonstrated prominent staining of putative gap junctions between fibroblasts. Temporal observations indicated that the interconnected cells and cellular processes undergo sequential positional changes leading to orientation of cells and interconnected cell processes parallel to the wound margin. Laser scanning confocal microscopy of en bloc, phalloidin-stained corneal wounds showed prominent intracellular f-actin bundles (i.e. stress fibres) within cell processes which formed an extensive interwoven pattern within the wound.