Macrophages and microvessel density in tumors of the ovary
Macrophages and microvessel density in tumors of the ovary
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DOI：
10.1006/gyno.1998.5292
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发表时间：
1999-04-01
影响因子：
4.7
通讯作者：
Rogers, PAW
中科院分区：
文献类型：
--
作者：
Orre, M;Rogers, PAW
巨噬细胞是单核巨噬细胞系统的成员，并合成和释放许多血管生成刺激剂和抑制剂。本研究的目的是比较不同类型卵巢肿瘤之间以及高（HVD）和平均（AVD）微血管密度（MVD）区域之间的巨噬细胞数量。使用CD 68抗体使巨噬细胞可视化。将先前确定的HVD和AVD区域定位在CD 68免疫染色切片上，并使用图像分析软件对每平方毫米阳性染色细胞的数量进行计数。CD 68阳性巨噬细胞数在卵巢良性肿瘤、粘液性肿瘤和浆液性肿瘤之间以及HVD和AVD区之间均无差异（ANOVA; P > 0.05）。在良性肿瘤的HVD和AVD区，巨噬细胞数与MVD呈正相关（斯皮尔曼等级相关系数; P < 0.03）。而在浆液性肿瘤的HVD区，巨噬细胞数与MVD呈显著负相关（斯皮尔曼等级相关系数，P > 0.05）。巨噬细胞浸润在卵巢肿瘤类型之间似乎没有差异;然而，巨噬细胞可能对良性卵巢肿瘤的血管形成有积极影响。(C)北京：科学出版社.
Macrophages are members of the mononuclear phagocyte system and synthesize and release many angiogenesis stimulators and inhibitors, Previous studies have demonstrated heterogeneous macrophage populations in ovarian tumors. The aim of this study was to compare macrophage number between different types of ovarian tumors and between regions of high (HVD) and average (AVD) microvessel density (MVD). Macrophages were visualized using CD68 antibody, Previously determined regions of HVD and AVD were located on CD68 immunostained sections and image analysis software was used to count the number of positively stained cells per square millimeter. CD68-positive macrophage number did not differ between benign, mucinous, and serous ovarian tumors or between regions of HVD and AVD (ANOVA; P > 0.05), In both HVD and AVD regions of benign tumors, macrophage number positively correlated with MVD (Spearman rank correlation coefficient; P < 0.03). In contrast, a small but significant negative correlation between macrophage number and MVD was observed in HVD regions of serous tumors (Spearman rank correlation coefficient; P > 0.05). Macrophage infiltration does not appear to differ between ovarian tumor types; however, macrophages may have a positive influence on the vascularization of benign ovarian tumors. (C) 1999 Academic Press.