Evaluation of the cytotoxicity and genotoxicity of curcumin in PC12 cells
Evaluation of the cytotoxicity and genotoxicity of curcumin in PC12 cells
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DOI：
10.1016/j.mrgentox.2009.02.003
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发表时间：
2009-04-30
影响因子：
1.9
通讯作者：
Greggi Antunes, Lusania Maria
中科院分区：
文献类型：
--
作者：
Mendonca, Leonardo Meneghin;dos Santos, Graciela Cristina;Greggi Antunes, Lusania Maria
活性氧诱导的神经毒性可表现为铂玉米粒化疗的不良反应。如顺铂。由于其不良反应，包括肾毒性、耳毒性，在临床实践中使用该药物受到限制。神经毒性和遗传毒性。在一些实验和模型中，功能性食品或营养保健品已经显示出潜在的神经保护活性。本研究旨在探讨姜黄素对暴露于顺铂的PC12细胞可能的细胞毒性和遗传毒性/抗基因毒性作用。分别采用MTT法和微核试验评价细胞活力和遗传毒性/抗遗传毒性。用不同浓度的顺铂和姜黄素（0.5 ~ 128 μ g/mL）处理PC12细胞。分析结果表明，与对照组相比，高浓度姜黄素具有细胞毒性，微核频率增加。在相关处理中，在所有三种浓度下进行评估。姜黄素显著降低顺铂诱导的微核总频率。在神经元模型中，确定这种常用抗氧化剂的细胞毒性和基因毒性/抗基因毒性作用对于评估与其他化学制剂（包括用于癌症治疗的化疗药物）联合使用时可能的危害非常重要。(C) 2009 Elsevier B.V.版权所有
Neurotoxicity induced by reactive oxygen species can appear as an adverse effect of chemotherapy treatment with platinum corn pounds. such as cisplatin. The use of this drug in clinical practice is limited due to its adverse effects, including nephrotoxicity, ototoxicity. neurotoxicity and genotoxicity. Functional foods or nutraceuticals have demonstrated potential neuroprotective activity in several experiments and models. This study aimed to investigate the possible cytotoxicity and genotoxicity/antigenotoxic effects of curcumin in PC12 cells exposed to cisplatin. Cell viability and genotoxicity/antigenotoxicity were evaluated by the MTT assay and micronucleus test, respectively. PC12 cells were treated with different concentrations of cisplatin and curcumin (0.5-128 mu g/mL). Analysis of the results showed that high concentrations of curcumin were cytotoxic and increased micronuclei frequency compared to the control group. In the associated treatments, at all three concentrations evaluated. curcumin significantly reduced the total frequency of micronuclei induced by cisplatin. Determining the cytotoxic and genotoxic/antigenotoxic effects of this frequently used antioxidant in a neuronal model is important to assess possible hazards when combined with other chemical agents, including chemotherapy drugs used in cancer therapy. (C) 2009 Elsevier B.V. All rights reserved.