MOLECULAR ASSOCIATION BETWEEN MAJOR HISTOCOMPATIBILITY COMPLEX CLASS-I ANTIGENS AND INSULIN-RECEPTORS IN MOUSE-LIVER MEMBRANES
MOLECULAR ASSOCIATION BETWEEN MAJOR HISTOCOMPATIBILITY COMPLEX CLASS-I ANTIGENS AND INSULIN-RECEPTORS IN MOUSE-LIVER MEMBRANES
复制标题
DOI：
10.1073/pnas.82.24.8634
复制
发表时间：
1985-12-01
影响因子：
11.1
通讯作者：
BROSSETTE, N
中科院分区：
文献类型：
--
作者：
FEHLMANN, M;PEYRON, JF;BROSSETTE, N
主要组织相容性复合体 (MHC) 抗原和细胞蛋白之间的分子关联被认为涉及 MHC 抗原的各种免疫学和非免疫学功能，包括激素信号传导。在同源 H-2k 小鼠的肝质膜中研究了胰岛素受体和 MHC I 类抗原之间存在的物理相互作用。胰岛素受体被 125I 标记的光反应性胰岛素类似物特异性标记，细胞蛋白被溶解并与各种单克隆抗体一起孵育。通过聚丙烯酰胺凝胶电泳随后进行放射自显影来分析免疫沉淀物。与 H-2k I 类抗原上的不同表位反应的抗体都能够在 H-2k 小鼠肝膜中沉淀高达 25% 的标记胰岛素受体，而在 H-2b 小鼠肝膜中没有沉淀胰岛素受体。连续免疫沉淀显示胰岛素受体和H-2抗原共沉淀并且没有发生交叉反应。通过光反应性胰岛素和乳过氧化物酶催化的碘化对膜蛋白进行双重标记，证明了胰岛素受体和 H-2 抗原之间相互作用的特异性。因此，这些结果表明，在小鼠肝膜中，胰岛素受体与 MHC 的 I 类抗原物理相关。
Molecular association between major histocompatibility complex (MHC) antigens and cellular proteins are thought to be involved in various immunological and nonimmunological functions of MHC antigens, including hormone signaling. The existence of physical interactions between insulin receptors and MHC class I antigens was ivestigated in liver plasma membranes form congenic H-2k mice. Insulin receptors were specifically labeled with a 125I-labeled photoreactive insulin analogue, and cellular proteins were solubilized and incubated with various monoclonal antibodies. Immunoprecipitates were analyzed by polyacrylamide gel electrophoresis followed by autoradiography. Antibodies reacting with distinct epitopes on H-2k class I antigens were all able to precipitate up to 25% of the labeled insulin receptors in H-2k mouse liver membranes, whereas no insulin receptors were precipitated in H-2b mouse liver membrnaes. Sequential immunoprecipitations showed that insulin receptors and H-2 antigens were coprecipitated and that no cross-reactivity occurred. The specificity of the interaction between insulin receptors and H-2 antigens was demonstrated after double labeling of membrane proteins by photoreactive insulin and lactoperoxidase-catalyzed iodination. These results thus show that, in mouse liver membranes, insulin receptors are physically associated to class I antigens of the MHC.