Serial propagation of human endothelial cells in vitro.
Serial propagation of human endothelial cells in vitro.
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体外人类内皮细胞的系列传播。
DOI：
10.1083/jcb.91.2.420
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发表时间：
1981-11
影响因子：
7.8
通讯作者：
Weinstein, R
中科院分区：
文献类型：
--
作者：
Maciag, T;Hoover, G A;Stemerman, M B;Weinstein, R
人脐静脉（HUV）内皮细胞在人纤连蛋白（HFN）基质上以1：5的分流比（至少27个群体倍增）在补充有胎牛血清（FBS）和内皮细胞生长因子（ECGF）的培养基199中生长15至21代。该系统还允许HUV内皮细胞在低至1.25个细胞/cm 2的细胞密度下生长。除了延缓HUV内皮细胞的过早衰老外，ECGF还降低了低密度HUV内皮细胞生长的血清需求;与高血清对照相比，2.5%血清和ECGF产生最大生长的一半。显著的HUV内皮细胞生长也观察到补充有羊垂体切除（HYPOX）血清，血浆来源的血清（PDS），或HYPOX-PDS的ECGF的存在下，在培养基中，这表明，无论是垂体或血小板有助于HUV内皮细胞的生长。
Human umbilical vein (HUV) endothelial cells were grown for 15 to 21 passages at a split ratio of 1:5 (at least 27 population doublings) on a human fibronectin (HFN) matrix in Medium 199 supplemented with fetal bovine serum (FBS) and endothelial-cell growth factor (ECGF). This system also permitted the growth of HUV endothelial cells at cell densities as low as 1.25 cells/cm2. In addition to delaying the premature senescence of HUV endothelial cells, ECGF also reduced the serum requirement for low-density HUV endothelial-cell growth; 2.5% serum and ECGF yields half-maximum growth as compared to high serum controls. Significant HUV endothelial-cell growth was also observed in medium supplemented with either ovine hypophysectomized (HYPOX) serum, plasma-derived serum (PDS), or HYPOX-PDS in the presence of ECGF, suggesting that neither the pituitary nor the platelet contributes to HUV endothelial-cell growth.