Monoclonal antibodies reveal expression of the CGRP receptor in Purkinje cells, interneurons and astrocytes of rat cerebellar cortex
Monoclonal antibodies reveal expression of the CGRP receptor in Purkinje cells, interneurons and astrocytes of rat cerebellar cortex
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单克隆抗体揭示大鼠小脑皮质浦肯野细胞、中间神经元和星形胶质细胞中 CGRP 受体的表达
作者：
S. Morara;S. Wimalawansa;A. Rosina
成年大鼠小脑分子层显示高水平的降钙素基因相关肽(CGRP)受体，但该受体的细胞定位尚不清楚。为了揭示这些受体的表达部位，用从猪小脑膜上提纯的CGRP受体的单抗，结合共聚焦显微镜进行了双免疫荧光实验。PEP-19是一种高度富含于浦肯野细胞(Pc)的标记物，发现CGRP受体位于Pc细胞质和树突中，在那里它们标记的小斑点有时沿着树突本身排列成一行。CGRP受体也位于抑制性中间神经元。此外，与GFAP双标实验表明，CGRP受体-IR标记高尔基体上皮细胞及其放射状纤维(Bergmann纤维)，以及围绕Pc Somata的星形细胞突起。CGRP受体同时存在于Purkinje细胞和包裹着Purkinje细胞的神经胶质细胞中，这使得我们可以假设这些受体可能参与神经元-神经胶质细胞之间的相互作用，影响神经元的活动。
THE molecular layer of adult rat cerebellum displays high levels of calcitonin gene-related peptide (CGRP) receptors, but the cellular location of the receptor remains unidentified. In an attempt to reveal the expression sites of these receptors, monoclonal antibodies raised against purified CGRP receptors from porcine cerebellar membranes were used in double-immunofluorescence experiments combined with confocal microscopy. PEP-19, a marker that is highly enriched in Purkinje cells (Pc), revealed that CGRP receptors are located in Pc cytoplasm and dendrites, where they label small puncta sometimes arranged in a row along the course of the dendrite itself. CGRP receptors were also located in inhibitory interneurons. Furthermore, as shown by double-labeling experiments with GFAP, CGRP receptor-IR labeled Golgi epithelial cells and their radial fibers (Bergmann fibers), as well as astrocytic processes encircling Pc somata. The simultaneous presence of CGRP receptors in Purkinje cells and in the glial cells that heavily enshroud Purkinje cells allows us to hypothesize that these receptors may be involved in neuron-glia interactions influencing neuronal activity.