Transcript identification and profiling during salt stress and recovery of Populus euphratica
Transcript identification and profiling during salt stress and recovery of Populus euphratica
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DOI：
10.1093/treephys/24.3.265
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发表时间：
2004-03-01
期刊：
影响因子：
4
通讯作者：
Pais, MS
中科院分区：
文献类型：
--
作者：
Gu, RS;Fonseca, S;Pais, MS
胡杨是一种耐盐物种，在水培条件下可以应对高达450 mM的氯化钠，在300 mM的氯化钠中生长时，可以容忍根和叶中高积累的Na+和Cl-。用抑制消减杂交法预先筛选出315个cDNA，用微阵列杂交技术检测盐胁迫和盐恢复后的转录反应。在加入300 mM的氯化钠后，热休克蛋白和富含羟脯氨酸的糖蛋白的转录物分别积累了1.5小时和3小时。盐胁迫显著上调的转录包括离子和渗透平衡元件，如镁转运蛋白、合成素蛋白、种子吸胀蛋白和质膜内源蛋白，代谢调节因子如细胞色素P450、锌指蛋白、裂解因子和转氨酶，以及光合作用激活酶Rubisco活化酶和光呼吸相关的乙醇酸氧化酶。一些与光合作用相关的转录本在72h的盐胁迫下表达下调，但在长期恢复(48h)后上调。蔗糖合成酶、ABC转运蛋白、钙调素、POP3多肽和水通道蛋白似乎积极参与植物从盐胁迫中恢复的过程。一些编码未知功能蛋白的转录本受到盐胁迫的调控。选择的转录本也用实时定量聚合酶链式反应进行了分析，这些转录本对盐胁迫的反应发生了变化。对该木本植物在盐胁迫和恢复过程中的转录分析表明，有几个基因和相应的蛋白质在今后的木本植物耐盐性研究中值得特别关注。
Populus euphratica Oli. is a salt-tolerant species that can cope with up to 450 mM NaCl under hydroponic conditions and can tolerate high accumulations of Na+ and Cl- in roots and leaves when grown in 300 mM NaCl. Transcript responses to salt stress and recovery were monitored by microarray hybridization of 315 cDNAs preselected by suppression subtractive hybridization. Transcripts of a heat-shock protein and a hydroxyproline-rich glycoprotein accumulated 1.5 and 3 h, respectively, after adding 300 mM NaCl to the culture medium. Transcripts significantly up-regulated by salt stress included ionic and osmotic homeostasis elements such as magnesium transporter-like protein, syntaxin-like protein, seed imbibition protein and plasma membrane intrinsic protein; metabolism regulators like cytochrome P450, zinc finger protein, cleavage factor and aminotransferase; and the photosynthesis-activating enzyme Rubisco activase and photorespiration-related glycolate oxidase. Several photosynthesis-related transcripts were down-regulated in response to 72 h of salt stress but were up-regulated after long-term recovery (48 h). Sucrose synthase, ABC transporter, calmodulin, Pop3 peptide and aquaporin appeared to be actively involved in the process of plant recovery from salt stress. Several transcripts encoding proteins of unknown function were regulated by salt stress. Selected transcripts exhibiting altered transcript profiles in response to salt stress were also analyzed by real-time quantitative PCR. Transcript analysis during salt stress and recovery of this woody species revealed several genes and corresponding proteins deserving special attention in future studies of salt tolerance in woody species.