On and off responses of the photopic fullfield ERG in normal subjects and glaucoma patients
On and off responses of the photopic fullfield ERG in normal subjects and glaucoma patients
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DOI：
10.1007/s10633-011-9258-1
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发表时间：
2011-02-01
影响因子：
1.4
通讯作者：
Kremers, Jan
中科院分区：
文献类型：
--
作者：
Horn, Folkert K.;Gottschalk, Kristina;Kremers, Jan
最近的研究表明，明视负反应（PhNR）与长时间的刺激的诊断价值。本研究的目的是记录正常人和青光眼患者的明视全视野视网膜电图（ERG）的开和关反应。我们重点研究了长时程刺激ERG的起始和终止后的不同反应波。在白色20 cd/m（2）背景上，记录响应于白色明亮LED闪光的明视觉全视野ERG。刺激亮度分别为40、60和80 cd/m2。使用闪光持续时间为240 ms和偏移时间为500 ms的平均响应。我们检查了19名健康受试者，27例青光眼性视盘萎缩和7高眼压患者。人的ERG的开和关反应的幅度和隐式时间依赖于闪光亮度。青光眼患者和健康受试者在60 cd/mA（2）闪光时，d波和i波的PhNR发生了显著变化（起始时：P < 0.01，终止时：P < 0.001），终止时i波的PhNR发生了显著变化（P < 0.01）。未发现全视野ERG的峰值时间以及a波和b波振幅有显著差异。PhNR振幅与OCT测量的视网膜神经纤维层的平均厚度显着相关。与正常明视长闪光ERG相比，青光眼患者显示刺激开始后的PhNR振幅和刺激偏移后的波的变化。
Recent studies suggest a diagnostic value of the photopic negative response (PhNR) with a long-duration stimulus. The aim of this study was to record the on and off responses of the photopic fullfield electroretinogram (ERG) in normal subjects and glaucoma patients. We focused on different waves of the responses after onset and offset of the long-duration stimulus ERG. Photopic fullfield ERGs were recorded in response to a white bright LED flash on a white 20 cd/m(2) background. Stimulus luminances were 40, 60 and 80 cd/m(2). Responses were averaged using a flash duration of 240 ms and an offset period of 500 ms. We examined 19 healthy subjects, 27 patients with glaucomatous optic disc atrophy and 7 ocular hypertensive patients. The amplitudes and implicit times of the on and off responses of the human ERG depended on flash luminance. Comparing patients with glaucoma and healthy subjects for the 60 cd/mA(2) flash, there was a significant change in the PhNRs (at onset: P < 0.01, at offset: P < 0.001) of the d-wave and of the i-wave at offset (P < 0.01). No significant difference was found for peak times of the fullfield ERG and for a- and b-wave amplitudes. PhNR amplitudes were significantly correlated with mean thickness of retinal nerve fibre layer as measured with OCT. In comparison with the normal photopic long-flash ERG, glaucoma patients showed changes in the PhNR amplitude following stimulus onset and in waves following stimulus offset.