Gastric cardia adenocarcinoma microRNA profiling in Chinese patients
Gastric cardia adenocarcinoma microRNA profiling in Chinese patients
复制标题
中国患者贲门腺癌 microRNA 分析
DOI：
10.1007/s13277-016-4824-5
复制
发表时间：
2016-07-01
期刊：
影响因子：
--
通讯作者：
Feng, Xiaoshan
中科院分区：
文献类型：
--
作者：
Gao, Shegan;Zhou, Fuyou;Feng, Xiaoshan
贲门腺癌（GCA），发生在胃食管交界处，是最恶性的癌症类型之一。在过去的30年中，GCA的发病率增加了约7倍，比许多其他恶性肿瘤的发病率增加更大。然而，由于以往的研究主要集中在非贲门部胃癌，到目前为止，GCA背后的机制仍然在很大程度上是未知的。microRNAs（miRNAs）在肿瘤发生中起着重要作用。为了深入了解miRNA在GCA发展中调控的分子机制，我们使用81对原发性GCA和相应的非致瘤组织研究了miRNA表达谱。首先，将21对样品用于微阵列分析，然后将另外60对样品用于进一步分析。结果显示，464个miRNAs（237个上调，227个下调，错误发现率FDR < 0.05）在GCA和非肿瘤组织中有差异表达。Pearson检验和通路分析表明，这些表达异常的miRNA相关编码RNA可能对多种肿瘤相关通路产生影响。发现四种miRNA（miR-1244、miR-135 b-5 p、miR-3196和miR-628- 3 p）与GCA分化相关。发现一种miRNA miR-196 a-5 p与GCA发病年龄相关。此外，生存分析显示miR-135 b-5 p的表达水平与GCA生存相关。综上所述，本研究首次提供了miRNA在GCA中的全基因组表达谱，为进一步的功能研究奠定了基础。
Gastric cardia adenocarcinoma (GCA), which occurs at the gastroesophageal boundary, is one of the most malignant types of cancer. Over the past 30 years, the incidence of GCA has increased by approximately sevenfold, which has a more substantial increase than that of many other malignancies. However, as previous studies mainly focus on non-cardia gastric cancer, until now, the mechanisms behind GCA remain largely unknown. MicroRNAs (miRNAs) have been shown to play pivotal roles in carcinogenesis. To gain insight into the molecular mechanisms regulated by miRNAs in GCA development, we investigated miRNA expression profiles using 81 pairs of primary GCAs and corresponding non-tumorigenic tissues. First, 21 pairs of samples were used for microarray analysis, and then another 60 pairs of samples were used for further analysis. Our results showed that 464 miRNAs (237 upregulated, 227 downregulated, false discovery rate FDR < 0.05) were differently expressed between GCA and non-tumor tissues. Pearson test and pathway analysis revealed that these dysregulated miRNA correlated coding RNAs may have effects on several cancer-related pathways. Four miRNAs (miR-1244, miR-135b-5p, miR-3196, and miR-628-3p) were found to be associated with GCA differentiation. One miRNA, miR-196a-5p, was found to be associated with age of GCA onset. Further, survival analysis showed that the expression level of miR-135b-5p was associated with GCA survival. Taken together, our study first provided the genome-wide expression profiles of miRNA in GCA and will be good help for further functional studies.