Drosophila melanogaster does not share the telomeric repeat sequence of another invertebrate, Ascaris lumbricoides.
Drosophila melanogaster does not share the telomeric repeat sequence of another invertebrate, Ascaris lumbricoides.
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黑腹果蝇不具有另一种无脊椎动物蛔虫的端粒重复序列。
DOI：
10.1007/bf00277145
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Levis,RW
中科院分区：
文献类型：
--
作者：
Levis,RW
所有真核生物染色体末端的DNA都含有一个特征性序列基序，该基序由规则或不规则重复单元的串联阵列组成。这些末端重复被认为是维持染色体末端所必需的。迄今为止研究的所有脊椎动物的末端重复序列是相同的，并且与高等植物和一些原生动物的末端重复序列足够相似，可以进行交叉杂交。然而，之前的研究尚未检测到黑腹果蝇的DNA与所测试的任何几种生物的末端DNA序列之间的交叉杂交。最近，第一个末端DNA克隆从多细胞无脊椎动物，即ofAscaris lumbricoides，据报道，也包括串联重复的短序列类似于以前确定的其他真核生物。在这里，我展示了这个序列的探针。蚯蚓不能与D.黑腹菌因此，与脊椎动物之间的保护相反，末端染色体序列似乎不是所有后生动物无脊椎动物所共有的。
The DNA at the chromosomal termini of all eukaryotes from which it has been isolated contains a characteristic sequence motif consisting of tandem arrays of a regular or irregular repeat unit. These terminal repeats are thought to be essential for the maintenance of the chromosome ends. The sequences of the terminal repeats of all vertebrates studied thus far are identical and are similar enough to those of higher plants and some protozoans to cross-hybridize. However, previous studies have not detected cross-hybridization between the DNA ofDrosophila mélanogasterand the terminal DNA sequences of any of several organisms tested. Recently, the first terminal DNA clone from a multicellular invertebrate, that ofAscaris lumbricoides, was reported also to consist of a tandem reiteration of a short sequence similar to those previously identified for other eukaryotes. Here I show that a probe for this sequence fromA. lumbricoidesfails to hybridize delectably to the DNA ofD. melanogaster. Thus, in contrast to their conservation among vertebrates, the terminal chromosomal sequences appear not to be shared by all metazoan invertebrates.