Two structurally analogous ruthenium complexes as naked-eye and reversible molecular "light switch" for G-quadruplex DNA
Two structurally analogous ruthenium complexes as naked-eye and reversible molecular "light switch" for G-quadruplex DNA
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DOI：
10.1016/j.jinorgbio.2014.07.003
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发表时间：
2014-11-01
影响因子：
3.9
通讯作者：
Yao, Tian-Ming
中科院分区：
文献类型：
--
作者：
Lu, Xiao-Hui;Shi, Shuo;Yao, Tian-Ming
合成并表征了一对对称的呋喃基钌配合物[Ru（phen）（2）dpq-df]（2+）（1）和[Ru（bpy）（2）dpq-df]（2+）（2）（phen = 1，10-菲咯啉，bpy = 2，2 ′-联吡啶，dpq-df =联吡啶并（3，2-a：2 ′，3 ′-c）喹喔啉-二呋喃）.通过荧光光谱、紫外-可见光谱、圆二色性（CD）、荧光共振能量转移（FRET）熔解分析和分子对接研究，研究了这两种配合物对G-四链体DNA的结合特性。实验结果表明，两种钌配合物在杂合G-四链体DNA的存在下均表现出明显的“光开关”效应。有趣的是，通过连续加入Cu ~（2+）离子和EDTA，这种“光开关”可以反复关闭和打开，而且这些行为甚至可以用肉眼观察到。此外，FRET熔解分析表明，这两种复合物可能是潜在的G-四链体结构的稳定剂。计算研究不仅证实了两个复合物分子与一个G-四链体DNA分子结合，而且解释了“光开关”效应。(C)2014爱思唯尔公司All rights reserved.
A pair of symmetrical furyl based ruthenium(II) complexes ([Ru(phen)(2)dpq-df](2+) (1) and [Ru(bpy)(2)dpq-df](2+) (2) (phen = 1,10-phenanthroline, bpy = 2,2'-bipyridine, dpq-df = dipyrido (3,2-a:2',3'-c) quinoxaline-difuran) have been prepared and characterized. The binding properties of both complexes toward G-quadruplex DNA have been investigated by fluorescence spectroscopy, UV-Vis spectroscopy, circular dichroism (CD), fluorescence resonance energy transfer (FRET) melting assays and molecular docking studies. The experimental results indicated that both Ru-complexes exhibited a remarkable "light switch" effect in the presence of hybrid G-quadruplex DNA Interestingly, the "light switch" can be repeated off and on through the successive addition of Cu2+ ions and EDTA, and all these behaviors can be observed even by the naked eyes. Moreover, FRET melting assay revealed that both complexes could be potential stabilizers for G-quadruplex architectures. The computational studies not only confirmed that the two complex molecules bound to one G-quadruplex DNA molecule, but also explained the "light switch" effect. (C) 2014 Elsevier Inc. All rights reserved.