Preparation of functionally PEGylated gold nanoparticles with narrow distribution through autoreduction of auric cation by α-biotinyl-PEG-block-[poly(2-(N,N-dimethylamino)ethyl methacrylate)]
Preparation of functionally PEGylated gold nanoparticles with narrow distribution through autoreduction of auric cation by α-biotinyl-PEG-block-[poly(2-(N,N-dimethylamino)ethyl methacrylate)]
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DOI：
10.1021/la035653i
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发表时间：
2004-02-03
期刊：
影响因子：
3.9
通讯作者：
Nagasaki, Y
中科院分区：
文献类型：
--
作者：
Ishii, T;Otsuka, H;Nagasaki, Y
采用α -生物素-PEG-嵌段聚[2-（N，N-二甲氨基）甲基丙烯酸乙酯]（生物素-PEG/PAMA）在室温水溶液中催化HAuCl4自动还原法制备了生物素基团位于PEG链末端的聚乙二醇化金纳米颗粒。通过改变初始Au3+/聚合物的比例，可以控制金纳米颗粒的尺寸在6 ~ 13 nm范围内，同时保持其狭窄的尺寸分布。即使在盐浓度高达I = 2.0的条件下，纳米颗粒在水介质中的分散稳定性也非常高。生物素- peg / pama锚定的金纳米颗粒在链霉亲和素存在下发生特异性聚集，揭示了它们作为生物条件下适用的胶体传感系统的前景。
PEGylated gold nanoparticles with biotin moieties installed at the distal end of the PEG tethered chains were prepared by the autoreduction of HAuCl4 catalyzed by alpha-biotinyl-PEG-block-poly[2-(N,N-dimethylamino)ethyl methacrylate] (biotinyl-PEG/PAMA) in aqueous medium at room temperature. The size of the gold nanoparticles was controllable in a range of 6-13 nm by changing the initial Au3+/polymer ratio, while retaining their narrow size distribution. The dispersion stability of the nanoparticles in aqueous medium was extremely high even under the condition of salt concentration as high as I = 2.0. Biotinyl-PEG/PAMA-anchored gold nanoparticles underwent specific aggregation in the presence of streptavidin, revealing their promising utility as colloidal sensing systems applicable under biological condition.