Effect of capsaicin on substance P and nerve growth factor in adjuvant arthritic rats
Effect of capsaicin on substance P and nerve growth factor in adjuvant arthritic rats
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DOI：
10.1016/s0304-3940(97)00458-8
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发表时间：
1997-07-11
影响因子：
2.5
通讯作者：
Tomlinson, DR
中科院分区：
文献类型：
--
作者：
Garrett, NE;Cruwys, SC;Tomlinson, DR
我们研究了辣椒素预处理（50mg kg(-1) s.c）对成年佐剂性关节炎大鼠P物质、前原激肽（PPT） mRNA和神经生长因子（NGF）及其高亲和力受体trkA的影响。佐剂性关节炎诱导21 d后，坐骨神经P物质水平显著升高，L-4/L-5背根神经节(DRG) γ - ppt mRNA和P物质水平虽小但不显著升高。关节炎21天后，坐骨神经和足部皮肤中的NGF水平以及DRG trkA mRNA没有变化，这表明NGF可能在慢性炎症中不起作用。辣椒素处理显著降低大鼠坐骨神经各组织P物质和NGF水平。辣椒素处理后，DRG中γ - ppt mRNA和trkA mRNA的表达量显著升高。本研究中使用的神经和皮肤组织来自同一只大鼠，我们之前已经证明辣椒素预处理显著减轻关节炎的严重程度（Cruwys, s.c., Garrett， N.E.和Kidd， b.l.，用辣椒素减轻关节炎大鼠的炎症和伤害感觉），神经科学。列托人。（1995） 205-207)。与辣椒素治疗的对照组大鼠相比，辣椒素治疗的关节炎大鼠对任何组织中的P物质或NGF水平没有影响，这表明感觉神经系统的药理学损伤可以减轻炎症性关节疾病的严重程度。(C) 1997爱思唯尔科学爱尔兰有限公司
We have investigated the effect of capsaicin pretreatment (50 mg kg(-1) s.c.) on substance P, preprotachykinin (PPT) mRNA, and nerve growth factor (NGF), plus its high-affinity receptor, trkA, in adult rats with adjuvant arthritis. Twenty one days after induction of adjuvant arthritis, sciatic nerve levels of substance P were significantly increased whilst there was a small but non-significant increase in gamma-PPT mRNA and substance P in L-4/L-5 dorsal root ganglia (DRG). NGF levels in sciatic nerve and foot skin as well as DRG trkA mRNA were unaltered after 21 days arthritis suggesting that NGF may not play a role in chronic inflammation. Capsaicin treatment of naive rats significantly reduced substance P in all tissues and NGF levels in the sciatic nerve. In contrast, gamma-PPT mRNA and trkA mRNA expression in DRG were significantly increased after capsaicin treatment. The nervous and skin tissues used in this study were harvested from the same rats in which we had previously shown that capsaicin pretreatment significantly attenuated the severity of arthritis (Cruwys, S.C., Garrett, N.E. and Kidd, B.L., Sensory denervation with capsaicin attenuates inflammation and nociception in arthritic rats, Neurosci. Lett., 193 (1995) 205-207). Arthritis in capsaicin-treated rats had no effect on substance P or NGF levels in any tissue when compared with capsaicin-treated control rats, suggesting that pharmacological impairment of the sensory nervous system can reduce the severity of inflammatory joint disease. (C) 1997 Elsevier Science Ireland Ltd.