Meiotic nuclear divisions in budding yeast require PP2A(Cdc55)-mediated antagonism of Net1 phosphorylation by Cdk.
Meiotic nuclear divisions in budding yeast require PP2A(Cdc55)-mediated antagonism of Net1 phosphorylation by Cdk.
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DOI：
10.1083/jcb.201103019
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发表时间：
2011-06-27
期刊：
影响因子：
--
通讯作者：
Arumugam P
中科院分区：
文献类型：
--
作者：
Kerr GW;Sarkar S;Tibbles KL;Petronczki M;Millar JB;Arumugam P
PP 2ACdc 55对抗Cdk对Net 1的磷酸化，以控制芽殖酵母中的减数分裂核分裂。在减数分裂期间，一轮脱氧核糖核酸复制之后是两轮核分裂。在酿酒酵母中，Cdc 14早期后期释放（FEAR）网络的激活是退出减数分裂I所必需的，但不会导致复制起点的激活。FEAR如何调节减数分裂的确切机制尚不清楚。在本文中，我们报告，过早激活的FEAR在减数分裂引起的蛋白磷酸酶PP 2ACdc 55活性的损失块双极纺锤体组装和核分裂。在cdc 55减数分裂无效（cdc 55-mn）细胞中，细胞周期蛋白依赖性激酶（Cdk）-抵消磷酸酶Cdc 14从核仁中过早释放伴随着其核仁锚蛋白Net 1的过度磷酸化。至关重要的是，缺乏6个Cdk磷酸化位点的Net 1突变形式挽救了cdc 55-mn细胞的减数分裂缺陷。CDC 14的显性突变等位基因的表达模拟了cdc 55-mn表型。我们认为PP 2ACdc 55对Net 1的磷酸化调节对于防止早熟退出减数分裂I是必不可少的。
PP2ACdc55 opposes the phosphorylation of Net1 by Cdk to control meiotic nuclear divisions in budding yeast. During meiosis, one round of deoxyribonucleic acid replication is followed by two rounds of nuclear division. In Saccharomyces cerevisiae, activation of the Cdc14 early anaphase release (FEAR) network is required for exit from meiosis I but does not lead to the activation of origins of replication. The precise mechanism of how FEAR regulates meiosis is not understood. In this paper, we report that premature activation of FEAR during meiosis caused by loss of protein phosphatase PP2ACdc55 activity blocks bipolar spindle assembly and nuclear divisions. In cdc55 meiotic null (cdc55-mn) cells, the cyclin-dependent kinase (Cdk)–counteracting phosphatase Cdc14 was released prematurely from the nucleolus concomitant with hyperphosphorylation of its nucleolar anchor protein Net1. Crucially, a mutant form of Net1 that lacks six Cdk phosphorylation sites rescued the meiotic defect of cdc55-mn cells. Expression of a dominant mutant allele of CDC14 mimicked the cdc55-mn phenotype. We propose that phosphoregulation of Net1 by PP2ACdc55 is essential for preventing precocious exit from meiosis I.
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