INFLUENCE OF CORTICOTROPIN-RELEASING FACTOR ON REPRODUCTIVE FUNCTIONS IN THE RAT
INFLUENCE OF CORTICOTROPIN-RELEASING FACTOR ON REPRODUCTIVE FUNCTIONS IN THE RAT
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DOI：
10.1210/endo-114-3-914
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发表时间：
1984-01-01
期刊：
影响因子：
4.8
通讯作者：
VALE, W
中科院分区：
文献类型：
--
作者：
RIVIER, C;VALE, W
将0.015-1.5 nmol绵羊促肾上腺皮质激素释放因子（CRF）急性注入性腺切除（或性腺切除/肾上腺切除）雌性大鼠的侧脑室，导致LH [促黄体激素]（但不是FSH）分泌的快速和长期剂量相关抑制。相比之下，急性外周注射高达15 nmol CRF在相同的动物制剂中没有效果。在骑自行车的完整雌性大鼠中，向大脑注射1.5 nmol CRF或皮下注射75 nmol CRF 抑制排卵并阻断约50%动物的发情前期LH峰。较低剂量的外周给药CRF无效。CRF每日皮下注射(15 nmol/天），导致40%的妊娠中断。CRF将降低血浆LH水平，并且在缺乏肾上腺或性腺来源的循环类固醇的情况下可以发挥这种作用。CRF对LH分泌的抑制作用不受阿片受体拮抗剂纳洛酮或神经节阻滞剂氯异山达明的影响。CRF诱导的β-受体阻滞剂地塞米松释放内啡肽或促肾上腺皮质激素进入体循环不干扰CRF对LH分泌的抑制作用。CRF显然通过大脑作用部位对生殖功能产生有害作用，至少在所用的实验设计下，这些部位似乎不直接涉及阿片或外周儿茶酚胺能途径。
The acute administration of 0.015-1.5 nmol ovine corticotropin-releasing factor (CRF) into the lateral ventricle of gonadectomized (or gonadectomized/adrenalectomized) female rats caused a rapid and prolonged dose-related inhibition of LH [luteinizing hormone] (but not FSH) secretion. By contrast, the acute peripheral injection of up to 15 nmol CRF was without effect in the same animal preparations. In cycling intact female rats, injection of 1.5 nmol CRF into the brain or of 75 nmol CRF s.c. inhibited ovulation and blocked the proestrous LH surge in about 50% of the animals. Lower doses of peripherally administered CRF were ineffective. CRF injected daily s.c. (15 nmol/day) to female rats during the first 12 days after mating caused a 40% disruption of pregnancy. CRF will lower plasma LH levels and can exert this effect in the absence of circulating steroids of either adrenal or gonadal origin. CRF inhibition of LH secretion was unaltered by the opiate receptor antagonist naltrexone or by the ganglionic blocker chlorisondamine. Blockade of CRF-induced .beta.-endorphin or ACTH release into the general circulation by dexamethasone did not interfere with the inhibitory effect of CRF on LH secretion. CRF apparently exerts deleterious actions on reproductive functions through brain sites of action which, at least under the experimental design used, do not appear to directly involve opiate or peripheral catecholaminergic pathways.