Turtle ghrelin.
Turtle ghrelin.
复制标题
龟胃饥饿素。
DOI：
10.1038/ng.2988
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发表时间：
2014
期刊：
影响因子：
30.8
通讯作者：
Guojie Zhang and Naoki Irie
中科院分区：
文献类型：
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作者：
Juan Pascual-Anaya;Amonida Zaddissa;Bronwen Aken;Guojie Zhang and Naoki Irie
Pascual-Anaya等回复：虽然我们基于对这些海龟基因组的生物信息学分析，报告了编码ghrelin的GHRL基因在海龟物种（Pelodiscus sinensis和Chelonia mydas）中丢失1，但我们现在已经证实GHRL基因确实存在于这些物种中。两项独立研究报告了这一发现，并对两种海龟基因组序列进行了详细的再分析2，3，这一发现得到了我们自己的基于PCR的检测方法的证实，在该方法中，我们克隆并测序了编码中华鳖成体组织中前促生长素原的cDNA（图1和补充说明）。相关Ensembl数据库已更新，以反映此更正（版本75，2014年2月;新记录ID ENSPSIT 00000022826;补充图1和2）。正如所预期的，尽管使用非标准化RT-PCR进行评价，但胃饥饿素表达在胃中较高（图1）。预测的蛋白质序列
Pascual-Anaya et al. reply: Although we reported that the GHRL gene encoding ghrelin was lost in turtle species (Pelodiscus sinensis and Chelonia mydas) on the basis of bioinformatics analysis of the genomes of these turtles1, we have now confirmed that GHRL genes actually exist in these species. This finding reported by two independent studies that performed detailed reanalysis of the two turtle genomic sequences2, 3 was confirmed by our own PCR-based detection method in which we cloned and sequenced the cDNA encoding preproghrelin in P. sinensis adult tissues (Fig. 1 and Supplementary Note). The related Ensembl database has been updated to reflect this corrigendum (release 75, February 2014; new transcript ID ENSPSIT00000022826; Supplementary Figs. 1 and 2). As expected, ghrelin expression was high in stomach, albeit evaluated using non-normalized RT-PCR (Fig. 1). The protein sequences predicted on