Mass Spectrometric Analysis of Mitochondrial RNA Modifications

Mass Spectrometric Analysis of Mitochondrial RNA Modifications
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线粒体 RNA 修饰的质谱分析

DOI:
10.1007/978-1-0716-0834-0_8
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发表时间:
2020
影响因子:
--
通讯作者:
Suzuki Takeo
Suzuki Takeo
中科院分区:
--
文献类型:
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作者:
Ishigami Yuma;Suzuki Tsutomu;Suzuki Takeo

文献摘要

相似文献

线粒体rna在转录后被修饰。这些修饰是RNA分子正常运作所必需的,因此有助于基本的线粒体过程。在此,我们描述了我们最新的基于质谱的平台,用于分析线粒体trna的转录后修饰,并测量线粒体rna修饰酶的体外活性。
Mitochondrial RNAs are modified posttranscriptionally. These modifications are required for proper functioning of RNA molecules, and thereby contribute to essential mitochondrial processes. Herein, we describe our latest mass spectrometry-based platform for analysis of posttranscriptional modifications of mitochondrial tRNAs, and measuring the in vitro activity of mitochondrial RNA-modifying enzymes.