Genomic insights into Aspergillus sydowii 29R-4-F02: unraveling adaptive mechanisms in subseafloor coal-bearing sediment environments.
Genomic insights into Aspergillus sydowii 29R-4-F02: unraveling adaptive mechanisms in subseafloor coal-bearing sediment environments.
复制标题
基因组洞察力对曲霉菌Sydowii 29R-4-F02：含潜层煤层沉积物环境中的自适应机制。
DOI：
10.3389/fmicb.2023.1216714
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发表时间：
2023
影响因子：
5.2
通讯作者：
中科院分区：
文献类型：
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作者：
曲霉是一种重要的丝状真菌，生活在不同的环境中。然而，对海底沉积物中A.sydowii的生物学和遗传学的研究仍然有限。在这里，我们进行了A.Sydowii 29R-4-F02基因组的从头测序和组装，该基因组是通过使用纳米孔测序平台从大约2.4公里深的2000万年海底含煤沉积物中获得的。测得基因组大小为37.19 Mb，GC含量为50.05%。最终组装由11个重叠群组成，N50为4.6 Mb，编码12,488个推测基因。值得注意的是，与陆地菌株CBS593.65相比，海底菌株29R-4-F02显示出更多的碳水化合物活性酶(CAZymes)以及与囊泡融合和自噬相关的独特基因。此外，在海底菌株29R-4-F02中还鉴定了257个阳性选择的基因，包括那些与DNA修复和CAZymes有关的基因。这些发现表明，A.Sydowii拥有一种独特的基因谱系，使其能够在极端的海底环境中生存数千万年。
Aspergillussydowii is an important filamentous fungus that inhabits diverse environments. However, investigations on the biology and genetics of A. sydowii in subseafloor sediments remain limited. Here, we performed de novo sequencing and assembly of the A. sydowii 29R-4-F02 genome, an isolate obtained from approximately 2.4 km deep, 20-million-year-old coal-bearing sediments beneath the seafloor by employing the Nanopore sequencing platform. The generated genome was 37.19 Mb with GC content of 50.05%. The final assembly consisted of 11 contigs with N50 of 4.6 Mb, encoding 12,488 putative genes. Notably, the subseafloor strain 29R-4-F02 showed a higher number of carbohydrate-active enzymes (CAZymes) and distinct genes related to vesicular fusion and autophagy compared to the terrestrial strain CBS593.65. Furthermore, 257 positively selected genes, including those involved in DNA repair and CAZymes were identified in subseafloor strain 29R-4-F02. These findings suggest that A. sydowii possesses a unique genetic repertoire enabling its survival in the extreme subseafloor environments over tens of millions of years.