Prevalence, viral load, and physical status of HPV 16 and 18 in cervical adenosquamous carcinoma
Prevalence, viral load, and physical status of HPV 16 and 18 in cervical adenosquamous carcinoma
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DOI：
10.1007/s00428-009-0823-x
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发表时间：
2009-09-01
期刊：
影响因子：
3.5
通讯作者：
Fukuda, Toshio
中科院分区：
文献类型：
--
作者：
Yoshida, Tomomi;Sano, Takaaki;Fukuda, Toshio
子宫颈腺鳞癌是一种罕见的恶性鳞状上皮和腺上皮成分的混合物，约占子宫颈癌的10%。本研究的目的是评估腺鳞癌中HPV 16和18的患病率、身体状况和病毒载量。对20例经病理证实的腺鳞癌组织进行福尔马林固定石蜡包埋。鳞状和腺体成分分别显微解剖和分析其HPV DNA亚型，病毒载量，并使用实时聚合酶链反应（PCR）的物理状态。HPV 16、18型阳性率分别为：鳞状上皮36.8%（7/19）、57.9%（11/19）;腺上皮33.3%（6/18）、61.1%（11/18）。E2引物PCR分析显示，11例HPV 18阳性病例中有7例（63.6%）在两个元件中均为纯整合形式。鳞状细胞中HPV 16 DNA拷贝数/细胞的平均值为7.22，腺体细胞中为1.33（p = 0.04），而相应的HPV 18 DNA拷贝数/细胞的平均值分别为1.50和0.89。HPV 18在腺鳞癌中的流行率很高，许多HPV 18阳性病例是纯整合形式，导致非常低的拷贝数/细胞。有可能是更积极的转化与HPV 18的早期整合导致更大的染色体不稳定性，更高的生长速度和快速进展的情况下。
Adenosquamous carcinoma of the uterine cervix is a rare mixture of malignant squamous and glandular epithelial elements and accounts for approximately 10% of cervical carcinomas. The aims of the present study were to evaluate the prevalence, physical status, and viral load of HPV 16 and 18 in adenosquamous carcinoma. Formalin-fixed paraffin-embedded tissue samples from 20 cases of histologically diagnosed adenosquamous carcinoma were examined. The squamous and glandular components were separately microdissected and analyzed for their HPV DNA subtype, viral load, and physical status using real-time polymerase chain reaction (PCR). The percentages of HPV 16- and 18-positive cases among all the HPV-positive cases were 36.8% (7/19) and 57.9% (11/19) in the squamous epithelial elements and 33.3% (6/18) and 61.1% (11/18) in the glandular elements, respectively. PCR analysis with E2 primers revealed that seven of eleven (63.6%) HPV 18-positive cases had the pure integrated form in both elements. The mean HPV 16 DNA copy numbers/cell was 7.22 in the squamous elements and 1.33 in the glandular elements (p = 0.04) while the corresponding mean HPV 18 DNA copy numbers/cell was 1.50 and 0.89, respectively. The prevalence of HPV 18 in adenosquamous carcinoma was high and many HPV 18-positive cases were the pure integrated form resulting in very low copy numbers/cell. It is possible that more aggressive transformation with early integration of HPV 18 results in cases with greater chromosomal instabilities, higher growth rates, and rapid progression.